Matches in SemOpenAlex for { <https://semopenalex.org/work/W4225262596> ?p ?o ?g. }
- W4225262596 endingPage "546" @default.
- W4225262596 startingPage "538" @default.
- W4225262596 abstract "Klebsiella pneumoniae is one of the major pathogens causing global multidrug-resistant infections. Therefore, strategies for preventing and controlling the infections are urgently needed. Phage depolymerase, often found in the tail fiber protein or the tail spike protein, is reported to have antibiofilm activity. In this study, phage P560 isolated from sewage showed specific for capsule locus type KL47 K. pneumoniae, and the enlarged haloes around plaques indicated that P560 encoded a depolymerase. The capsule depolymerase, ORF43, named P560dep, derived from phage P560 was expressed, purified, characterized and evaluated for enzymatic activity as well as specificity. We reported that the capsule depolymerase P560dep, can digest the capsule polysaccharides on the surface of KL47 type K. pneumoniae, and the depolymerization spectrum of P560dep matched to the host range of phage P560, KL47 K. pneumoniae. Crystal violet staining assay showed that P560dep was able to significantly inhibit biofilm formation. Further, a single dose (50 μg/mouse) of depolymerase intraperitoneal injection protected 90%-100% of mice from lethal challenge before or after infection by KL47 carbapenem-resistant K. pneumoniae. And pathological changes were alleviated in lung and liver of mice infected by KL47 type K. pneumoniae. It is demonstrated that depolymerase P560dep as an attractive antivirulence agent represents a promising tool for antimicrobial therapy." @default.
- W4225262596 created "2022-05-04" @default.
- W4225262596 creator A5011220640 @default.
- W4225262596 creator A5012324763 @default.
- W4225262596 creator A5015093553 @default.
- W4225262596 creator A5040090704 @default.
- W4225262596 creator A5055189263 @default.
- W4225262596 creator A5063514061 @default.
- W4225262596 creator A5067923607 @default.
- W4225262596 creator A5075716358 @default.
- W4225262596 creator A5082515921 @default.
- W4225262596 creator A5090366405 @default.
- W4225262596 date "2022-08-01" @default.
- W4225262596 modified "2023-10-04" @default.
- W4225262596 title "Identification of a phage-derived depolymerase specific for KL47 capsule of Klebsiella pneumoniae and its therapeutic potential in mice" @default.
- W4225262596 cites W1803102843 @default.
- W4225262596 cites W1902275581 @default.
- W4225262596 cites W1951178169 @default.
- W4225262596 cites W1983175129 @default.
- W4225262596 cites W1994831311 @default.
- W4225262596 cites W1995571921 @default.
- W4225262596 cites W2000443479 @default.
- W4225262596 cites W2002841189 @default.
- W4225262596 cites W2004548026 @default.
- W4225262596 cites W2019197162 @default.
- W4225262596 cites W2019795239 @default.
- W4225262596 cites W2041249082 @default.
- W4225262596 cites W2064289542 @default.
- W4225262596 cites W2068345774 @default.
- W4225262596 cites W2070300719 @default.
- W4225262596 cites W2088022009 @default.
- W4225262596 cites W2088527369 @default.
- W4225262596 cites W2098275718 @default.
- W4225262596 cites W2111533008 @default.
- W4225262596 cites W2119445362 @default.
- W4225262596 cites W2120902911 @default.
- W4225262596 cites W2121055574 @default.
- W4225262596 cites W2121917249 @default.
- W4225262596 cites W2122292306 @default.
- W4225262596 cites W2132849234 @default.
- W4225262596 cites W2145069900 @default.
- W4225262596 cites W2154066176 @default.
- W4225262596 cites W2154984047 @default.
- W4225262596 cites W2160719299 @default.
- W4225262596 cites W2189116376 @default.
- W4225262596 cites W2271991921 @default.
- W4225262596 cites W2323684023 @default.
- W4225262596 cites W2558551622 @default.
- W4225262596 cites W2579332158 @default.
- W4225262596 cites W2601382852 @default.
- W4225262596 cites W2605606775 @default.
- W4225262596 cites W2607964919 @default.
- W4225262596 cites W2619559306 @default.
- W4225262596 cites W2731127457 @default.
- W4225262596 cites W2736165363 @default.
- W4225262596 cites W2750630201 @default.
- W4225262596 cites W2761609060 @default.
- W4225262596 cites W2769071465 @default.
- W4225262596 cites W2802601005 @default.
- W4225262596 cites W2804291518 @default.
- W4225262596 cites W2807189395 @default.
- W4225262596 cites W2886418331 @default.
- W4225262596 cites W2897531714 @default.
- W4225262596 cites W2912170753 @default.
- W4225262596 cites W2914159229 @default.
- W4225262596 cites W2920280845 @default.
- W4225262596 cites W2942643736 @default.
- W4225262596 cites W2993639552 @default.
- W4225262596 cites W3005880153 @default.
- W4225262596 cites W3006611454 @default.
- W4225262596 cites W3007026683 @default.
- W4225262596 cites W3042934380 @default.
- W4225262596 cites W3043690282 @default.
- W4225262596 cites W3082156002 @default.
- W4225262596 cites W3091220734 @default.
- W4225262596 cites W3094306223 @default.
- W4225262596 cites W3133955161 @default.
- W4225262596 cites W3175060602 @default.
- W4225262596 cites W3177111506 @default.
- W4225262596 cites W3186601043 @default.
- W4225262596 cites W3187869602 @default.
- W4225262596 cites W3203419151 @default.
- W4225262596 cites W3205584802 @default.
- W4225262596 cites W3208862270 @default.
- W4225262596 cites W4211107894 @default.
- W4225262596 cites W4244116159 @default.
- W4225262596 cites W4254263948 @default.
- W4225262596 doi "https://doi.org/10.1016/j.virs.2022.04.005" @default.
- W4225262596 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35513275" @default.
- W4225262596 hasPublicationYear "2022" @default.
- W4225262596 type Work @default.
- W4225262596 citedByCount "14" @default.
- W4225262596 countsByYear W42252625962022 @default.
- W4225262596 countsByYear W42252625962023 @default.
- W4225262596 crossrefType "journal-article" @default.
- W4225262596 hasAuthorship W4225262596A5011220640 @default.
- W4225262596 hasAuthorship W4225262596A5012324763 @default.
- W4225262596 hasAuthorship W4225262596A5015093553 @default.