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• W4225278391 abstract "Consolidated bioprocessing (CBP) of lignocellulosic biomass to L-lactic acid using thermophilic cellulolytic/hemicellulolytic bacteria provides a promising solution for efficient lignocellulose conversion without the need for additional cellulolytic/hemicellulolytic enzymes. Most studies on the mesophilic and thermophilic CBP of lignocellulose to lactic acid concentrate on cultivation of non-cellulolytic mesophilic and thermophilic bacteria at temperatures of 30-55 °C with external addition of cellulases/hemicellulases for saccharification of substrates.L-Lactic acid was generated by fermenting microcrystalline cellulose or lignocellulosic substrates with a novel thermophilic anaerobic bacterium Caldicellulosiruptor sp. DIB 104C without adding externally produced cellulolytic/hemicellulolytic enzymes. Selection of this novel bacterium strain for lactic acid production is described as well as the adaptive evolution towards increasing the L-lactic acid concentration from 6 to 70 g/l on microcrystalline cellulose. The evolved strains grown on microcrystalline cellulose show a maximum lactic acid production rate of 1.0 g/l*h and a lactic acid ratio in the total organic fermentation products of 96 wt%. The enantiomeric purity of the L-lactic acid generated is 99.4%. In addition, the lactic acid production by these strains on several other types of cellulose and lignocellulosic feedstocks is also reported.The evolved strains originating from Caldicellulosiruptor sp. DIB 104C were capable of producing unexpectedly large amounts of L-lactic acid from microcrystalline cellulose in fermenters. These strains produce L-lactic acid also from lignocellulosic feedstocks and thus represent an ideal starting point for development of a highly integrated commercial L-lactic acid production process from such feedstocks." @default.
• W4225278391 created "2022-05-04" @default.
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• W4225278391 date "2022-05-02" @default.
• W4225278391 modified "2023-10-06" @default.
• W4225278391 title "Direct conversion of cellulose to l-lactic acid by a novel thermophilic Caldicellulosiruptor strain" @default.
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• W4225278391 doi "https://doi.org/10.1186/s13068-022-02137-7" @default.
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