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- W4225286335 endingPage "167620" @default.
- W4225286335 startingPage "167620" @default.
- W4225286335 abstract "Allosteric regulation of proteins has been utilized to study various aspects of cell signaling, from unicellular events to organism-wide phenotypes. However, traditional methods of allosteric regulation, such as constitutively active mutants and inhibitors, lack tight spatiotemporal control. This often leads to unintended signaling consequences that interfere with data interpretation. To overcome these obstacles, researchers employed protein engineering approaches that enable tight control of protein function through allosteric mechanisms. These methods provide high specificity as well as spatial and temporal precision in regulation of protein activity in vitro and in vivo. In this review, we focus on the recent advancements in engineered allosteric regulation and discuss the various bioengineered allosteric techniques available now, from chimeric GPCRs to chemogenetic and optogenetic switches. We highlight the benefits and pitfalls of each of these techniques as well as areas in which future improvements can be made. Additionally, we provide a brief discussion on implementation of engineered allosteric regulation approaches, demonstrating that these tools can shed light on elusive biological events and have the potential to be utilized in precision medicine." @default.
- W4225286335 created "2022-05-05" @default.
- W4225286335 creator A5026729056 @default.
- W4225286335 creator A5028541457 @default.
- W4225286335 creator A5059092669 @default.
- W4225286335 creator A5071448123 @default.
- W4225286335 date "2022-09-01" @default.
- W4225286335 modified "2023-10-10" @default.
- W4225286335 title "Engineered Allosteric Regulation of Protein Function" @default.
- W4225286335 cites W1484294450 @default.
- W4225286335 cites W1574582895 @default.
- W4225286335 cites W1598030966 @default.
- W4225286335 cites W1718009280 @default.
- W4225286335 cites W1774228708 @default.
- W4225286335 cites W1886963552 @default.
- W4225286335 cites W1894461759 @default.
- W4225286335 cites W1966403223 @default.
- W4225286335 cites W1968091681 @default.
- W4225286335 cites W1968442678 @default.
- W4225286335 cites W1969077273 @default.
- W4225286335 cites W1975563117 @default.
- W4225286335 cites W1982409013 @default.
- W4225286335 cites W1984175146 @default.
- W4225286335 cites W1985309852 @default.
- W4225286335 cites W1988840491 @default.
- W4225286335 cites W1990622714 @default.
- W4225286335 cites W1992639113 @default.
- W4225286335 cites W1999774383 @default.
- W4225286335 cites W2000046288 @default.
- W4225286335 cites W2006560015 @default.
- W4225286335 cites W2013726100 @default.
- W4225286335 cites W2018275031 @default.
- W4225286335 cites W2026911129 @default.
- W4225286335 cites W2027387232 @default.
- W4225286335 cites W2031264524 @default.
- W4225286335 cites W2039874742 @default.
- W4225286335 cites W2044476679 @default.
- W4225286335 cites W2046032580 @default.
- W4225286335 cites W2047567287 @default.
- W4225286335 cites W2050140768 @default.
- W4225286335 cites W2053704624 @default.
- W4225286335 cites W2056403651 @default.
- W4225286335 cites W2059002026 @default.
- W4225286335 cites W2063408699 @default.
- W4225286335 cites W2081758144 @default.
- W4225286335 cites W2082668329 @default.
- W4225286335 cites W2084427297 @default.
- W4225286335 cites W2087879073 @default.
- W4225286335 cites W2088942336 @default.
- W4225286335 cites W2096165020 @default.
- W4225286335 cites W2102463040 @default.
- W4225286335 cites W2104313183 @default.
- W4225286335 cites W2105929855 @default.
- W4225286335 cites W2112940322 @default.
- W4225286335 cites W2118536789 @default.
- W4225286335 cites W2119809698 @default.
- W4225286335 cites W2124990349 @default.
- W4225286335 cites W2132511722 @default.
- W4225286335 cites W2144473795 @default.
- W4225286335 cites W2157605186 @default.
- W4225286335 cites W2159828012 @default.
- W4225286335 cites W2167392460 @default.
- W4225286335 cites W2201429615 @default.
- W4225286335 cites W2221897369 @default.
- W4225286335 cites W2275148408 @default.
- W4225286335 cites W2283716387 @default.
- W4225286335 cites W2294945016 @default.
- W4225286335 cites W2319981452 @default.
- W4225286335 cites W2420468977 @default.
- W4225286335 cites W2437727589 @default.
- W4225286335 cites W2471853642 @default.
- W4225286335 cites W2472466887 @default.
- W4225286335 cites W2513066930 @default.
- W4225286335 cites W2516844665 @default.
- W4225286335 cites W2519539312 @default.
- W4225286335 cites W2520075163 @default.
- W4225286335 cites W2552634635 @default.
- W4225286335 cites W2562775433 @default.
- W4225286335 cites W2567498952 @default.
- W4225286335 cites W2579304123 @default.
- W4225286335 cites W2593384078 @default.
- W4225286335 cites W2597332893 @default.
- W4225286335 cites W2604304370 @default.
- W4225286335 cites W2736838547 @default.
- W4225286335 cites W2765153686 @default.
- W4225286335 cites W2767642053 @default.
- W4225286335 cites W2770485188 @default.
- W4225286335 cites W2782382515 @default.
- W4225286335 cites W2792102013 @default.
- W4225286335 cites W2803484441 @default.
- W4225286335 cites W2803649094 @default.
- W4225286335 cites W2808632617 @default.
- W4225286335 cites W2810768392 @default.
- W4225286335 cites W2883465913 @default.
- W4225286335 cites W2893090773 @default.
- W4225286335 cites W2897444944 @default.
- W4225286335 cites W2899563546 @default.
- W4225286335 cites W2908607257 @default.