Matches in SemOpenAlex for { <https://semopenalex.org/work/W4225331251> ?p ?o ?g. }
- W4225331251 abstract "In metazoans, a ≈1 megadalton (MDa) multiprotein complex comprising the dynein-dynactin adaptor Spindly and the ROD-Zwilch-ZW10 (RZZ) complex is the building block of a fibrous biopolymer, the kinetochore fibrous corona. The corona assembles on mitotic kinetochores to promote microtubule capture and spindle assembly checkpoint (SAC) signaling. We report here a high-resolution cryo-EM structure that captures the essential features of the RZZ complex, including a farnesyl-binding site required for Spindly binding. Using a highly predictive in vitro assay, we demonstrate that the SAC kinase MPS1 is necessary and sufficient for corona assembly at supercritical concentrations of the RZZ-Spindly (RZZS) complex, and describe the molecular mechanism of phosphorylation-dependent filament nucleation. We identify several structural requirements for RZZS polymerization in rings and sheets. Finally, we identify determinants of kinetochore localization and corona assembly of Spindly. Our results describe a framework for the long-sought-for molecular basis of corona assembly on metazoan kinetochores." @default.
- W4225331251 created "2022-05-05" @default.
- W4225331251 creator A5006549305 @default.
- W4225331251 creator A5016245786 @default.
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- W4225331251 creator A5053498446 @default.
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- W4225331251 creator A5073374028 @default.
- W4225331251 creator A5073503402 @default.
- W4225331251 creator A5080122227 @default.
- W4225331251 date "2022-04-04" @default.
- W4225331251 modified "2023-10-05" @default.
- W4225331251 title "Structure of the RZZ complex and molecular basis of Spindly‐driven corona assembly at human kinetochores" @default.
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