FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4225381331> ?p ?o ?g. }

• W4225381331 abstract "Cellular senescence, one of the hallmarks of aging, refers to a permanent cell cycle arrest and is accelerated during the aging process. A senolytic, a small molecule that eliminates senescent cells, can be a potential strategy to extend healthy lifespan and ameliorate age-associated diseases. Black ginseng (BG) prepared via nine-times repetitive steaming and drying process from fresh ginseng (Panax ginsengC.A. Meyer) has been reported to its physiological benefits against reactive oxygen species (ROS), inflammation, and oncogenesis which are common cues to induce aging. Therefore, the present study was aimed to investigate the effect of BG on cellular senescence.Senescence-associated β-galactosidase (SA-β-gal) staining was performed on primary mouse embryonic fibroblasts (MEFs) treated 5 μg/mL BG extract for 7 days, and then senescence was induced by γ-ray irradiation (20 Gy). For animal studies, C57BL/6 male mice were divided into three groups: 9-week-old mice orally administered distilled water (Young), 21-month-old mice received administration of distilled water (Old), and aged mice supplemented 300 mg/kg BG extracts (Old + BG). After 4 weeks of daily injection, PCR array and immunoblotting were conducted to evaluate mRNA expression and protein levels of targets associated with cellular senescence and cell cycle regulation in metabolic organs, liver, skeletal muscle (SKM), and white adipose tissue (WAT).In in vitro study, BG treatment reduced the density of SA-β-gal-positive MEFs after γ-ray irradiation (Fig. 1). In aged mice study, BG supplementation remarkedly downregulated age-related hepatic genes, especially in complement component 1q B (C1qb) and C (C1qc), compared to the aged control group. We further examined the canonical Wnt signaling, augmented in a mouse model of accelerated aging, since the complement C1q family activates Wnt signaling by binding to Frizzled receptors. By assessing the related markers, BG supplementation significantly decreased the hepatic β-catenin expression level; it also slightly reduced the levels of glycogen synthase kinase 3β (GSK3β) and its phosphorylated form (p-GSK3β). Recent studies have revealed that Wnt signaling involves in mammalian aging by regulating cell fate and proliferation choice. Thus, we evaluated the expressions of p53 and p21, which involves in cell cycle regulation, and both levels decreased in liver samples from Old + BG group compared to the Old group (Fig. 2). In support of this notion, BG treatment decreased the hepatic mRNA expression levels of senescence-associated secretory phenotypes (SASPs) such as matrix metallopeptidase 12 (Mmp12) and chemokine ligand 2 (Cxcl2). Consistent with the results in the liver, other metabolic organs, SKM and WAT, showed significantly lower p53 expression in Old + BG group over the Old group.The current study indicates that BG could be a potential candidate of senolytics to reversely regulate cellular senescence, resulting from downregulated complement system affecting Wnt signaling pathways and p53/p21 pathway in metabolic organs." @default.
• W4225381331 created "2022-05-05" @default.
• W4225381331 creator A5000777749 @default.
• W4225381331 creator A5027805612 @default.
• W4225381331 creator A5029935195 @default.
• W4225381331 creator A5030016314 @default.
• W4225381331 creator A5061810588 @default.
• W4225381331 date "2022-05-01" @default.
• W4225381331 modified "2023-09-27" @default.
• W4225381331 title "Black ginseng ameliorates cellular senescence via p53/p21 pathway" @default.
• W4225381331 doi "https://doi.org/10.1096/fasebj.2022.36.s1.r5925" @default.
• W4225381331 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35556451" @default.
• W4225381331 hasPublicationYear "2022" @default.
• W4225381331 type Work @default.
• W4225381331 citedByCount "0" @default.
• W4225381331 crossrefType "journal-article" @default.
• W4225381331 hasAuthorship W4225381331A5000777749 @default.
• W4225381331 hasAuthorship W4225381331A5027805612 @default.
• W4225381331 hasAuthorship W4225381331A5029935195 @default.
• W4225381331 hasAuthorship W4225381331A5030016314 @default.
• W4225381331 hasAuthorship W4225381331A5061810588 @default.
• W4225381331 hasConcept C104317684 @default.
• W4225381331 hasConcept C134018914 @default.
• W4225381331 hasConcept C142724271 @default.
• W4225381331 hasConcept C16685009 @default.
• W4225381331 hasConcept C203014093 @default.
• W4225381331 hasConcept C204787440 @default.
• W4225381331 hasConcept C2776151105 @default.
• W4225381331 hasConcept C2776914184 @default.
• W4225381331 hasConcept C2779232237 @default.
• W4225381331 hasConcept C48349386 @default.
• W4225381331 hasConcept C522857546 @default.
• W4225381331 hasConcept C55493867 @default.
• W4225381331 hasConcept C555283112 @default.
• W4225381331 hasConcept C71924100 @default.
• W4225381331 hasConcept C86803240 @default.
• W4225381331 hasConcept C95444343 @default.
• W4225381331 hasConceptScore W4225381331C104317684 @default.
• W4225381331 hasConceptScore W4225381331C134018914 @default.
• W4225381331 hasConceptScore W4225381331C142724271 @default.
• W4225381331 hasConceptScore W4225381331C16685009 @default.
• W4225381331 hasConceptScore W4225381331C203014093 @default.
• W4225381331 hasConceptScore W4225381331C204787440 @default.
• W4225381331 hasConceptScore W4225381331C2776151105 @default.
• W4225381331 hasConceptScore W4225381331C2776914184 @default.
• W4225381331 hasConceptScore W4225381331C2779232237 @default.
• W4225381331 hasConceptScore W4225381331C48349386 @default.
• W4225381331 hasConceptScore W4225381331C522857546 @default.
• W4225381331 hasConceptScore W4225381331C55493867 @default.
• W4225381331 hasConceptScore W4225381331C555283112 @default.
• W4225381331 hasConceptScore W4225381331C71924100 @default.
• W4225381331 hasConceptScore W4225381331C86803240 @default.
• W4225381331 hasConceptScore W4225381331C95444343 @default.
• W4225381331 hasIssue "S1" @default.
• W4225381331 hasLocation W42253813311 @default.
• W4225381331 hasLocation W42253813312 @default.
• W4225381331 hasOpenAccess W4225381331 @default.
• W4225381331 hasPrimaryLocation W42253813311 @default.
• W4225381331 hasRelatedWork W1967423388 @default.
• W4225381331 hasRelatedWork W2044156224 @default.
• W4225381331 hasRelatedWork W2064287125 @default.
• W4225381331 hasRelatedWork W2297447836 @default.
• W4225381331 hasRelatedWork W2318381739 @default.
• W4225381331 hasRelatedWork W2765102721 @default.
• W4225381331 hasRelatedWork W2897637407 @default.
• W4225381331 hasRelatedWork W2905201612 @default.
• W4225381331 hasRelatedWork W4293686155 @default.
• W4225381331 hasRelatedWork W60633371 @default.
• W4225381331 hasVolume "36" @default.
• W4225381331 isParatext "false" @default.
• W4225381331 isRetracted "false" @default.
• W4225381331 workType "article" @default.