FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4225388925> ?p ?o ?g. }

• W4225388925 endingPage "114539" @default.
• W4225388925 startingPage "114539" @default.
• W4225388925 abstract "Epithelial tissue or vesicular fluid from an unruptured or recently ruptured vesicle is the sample of choice for confirmatory laboratory diagnosis of foot-and-mouth disease (FMD). However, in ‘FMD-free’ countries the transport and downstream processing of such samples from potentially infected animals present a biosafety risk, particularly during heightened surveillance, potentially involving decentralised testing in laboratories without adequate biocontainment facilities. In such circumstances, rapid inactivation of virus, if present, prior to transport becomes a necessity, while still maintaining the integrity of diagnostic analytes. Tongue epithelium collected from cattle infected with FMD virus (FMDV) of serotype O (O/ALG/3/2014 – Lineage O/ME-SA/Ind-2001d) or A (A/IRN/22/2015 – Lineage A/ASIA/G-VII) was incubated in the PAXGene Tissue System Fixative (pH 4) and Stabiliser (pH 6.5) components respectively, in McIlvaine’s citrate-phosphate buffer (pH 2.6) or in phosphate-buffered saline (PBS, pH 7.4) at room temperature for 2, 6, 24 or 48 h. Following incubation, tissues were homogenised and tested by virus isolation and titration using LFBKαVβ6 cells. The integrity of FMD viral RNA was assessed by RT-qPCR (3Dpol coding region), Sanger sequencing of the VP1 region and transfection of LFBKαVβ6 cells to recover infectious virus. Viable virus could be recovered from samples incubated in PBS for at least 48 h. The PAXgene Tissue System Stabiliser component yielded variable results dependent on virus serotype, requiring at least 6 h of incubation to inactivate A/IRN/22/2015 in most samples, whereas the Fixative component required up to 2 h in some samples. McIlvaine’s citrate-phosphate buffer rapidly inactivated both viruses within 2 h of incubation. There was no demonstrable degradation of FMD viral RNA resulting from incubation in any of the buffers for up to 48 h, as assessed by RT-qPCR, and 24 h by sequencing and transfection to recover infectious virus. McIlvaine’s citrate-phosphate buffer (pH 2.6) is easy to prepare, inexpensive and inactivates serotype A and O FMDV in epithelial tissue within 2 h, while maintaining RNA integrity for downstream diagnostic processes and virus characterisation." @default.
• W4225388925 created "2022-05-05" @default.
• W4225388925 creator A5002774542 @default.
• W4225388925 creator A5009760007 @default.
• W4225388925 creator A5025370999 @default.
• W4225388925 creator A5051827363 @default.
• W4225388925 creator A5081763388 @default.
• W4225388925 date "2022-07-01" @default.
• W4225388925 modified "2023-10-14" @default.
• W4225388925 title "Chemical inactivation of foot-and-mouth disease virus in bovine tongue epithelium for safe transport and downstream processing" @default.
• W4225388925 cites W1801216971 @default.
• W4225388925 cites W1989069389 @default.
• W4225388925 cites W1998148929 @default.
• W4225388925 cites W2031371348 @default.
• W4225388925 cites W2040156022 @default.
• W4225388925 cites W2081000303 @default.
• W4225388925 cites W2119131783 @default.
• W4225388925 cites W2158018400 @default.
• W4225388925 cites W2315818384 @default.
• W4225388925 cites W2323438219 @default.
• W4225388925 cites W2583749971 @default.
• W4225388925 cites W2607032782 @default.
• W4225388925 cites W2793536687 @default.
• W4225388925 cites W2967671019 @default.
• W4225388925 cites W2988519919 @default.
• W4225388925 cites W3005146085 @default.
• W4225388925 cites W4231756443 @default.
• W4225388925 cites W57173378 @default.
• W4225388925 doi "https://doi.org/10.1016/j.jviromet.2022.114539" @default.
• W4225388925 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35523370" @default.
• W4225388925 hasPublicationYear "2022" @default.
• W4225388925 type Work @default.
• W4225388925 citedByCount "3" @default.
• W4225388925 countsByYear W42253889252022 @default.
• W4225388925 countsByYear W42253889252023 @default.
• W4225388925 crossrefType "journal-article" @default.
• W4225388925 hasAuthorship W4225388925A5002774542 @default.
• W4225388925 hasAuthorship W4225388925A5009760007 @default.
• W4225388925 hasAuthorship W4225388925A5025370999 @default.
• W4225388925 hasAuthorship W4225388925A5051827363 @default.
• W4225388925 hasAuthorship W4225388925A5081763388 @default.
• W4225388925 hasBestOaLocation W42253889251 @default.
• W4225388925 hasConcept C159047783 @default.
• W4225388925 hasConcept C2522874641 @default.
• W4225388925 hasConcept C25642318 @default.
• W4225388925 hasConcept C2776151397 @default.
• W4225388925 hasConcept C2779070877 @default.
• W4225388925 hasConcept C55493867 @default.
• W4225388925 hasConcept C7623868 @default.
• W4225388925 hasConcept C86803240 @default.
• W4225388925 hasConceptScore W4225388925C159047783 @default.
• W4225388925 hasConceptScore W4225388925C2522874641 @default.
• W4225388925 hasConceptScore W4225388925C25642318 @default.
• W4225388925 hasConceptScore W4225388925C2776151397 @default.
• W4225388925 hasConceptScore W4225388925C2779070877 @default.
• W4225388925 hasConceptScore W4225388925C55493867 @default.
• W4225388925 hasConceptScore W4225388925C7623868 @default.
• W4225388925 hasConceptScore W4225388925C86803240 @default.
• W4225388925 hasFunder F4320320464 @default.
• W4225388925 hasFunder F4320328984 @default.
• W4225388925 hasLocation W42253889251 @default.
• W4225388925 hasLocation W42253889252 @default.
• W4225388925 hasOpenAccess W4225388925 @default.
• W4225388925 hasPrimaryLocation W42253889251 @default.
• W4225388925 hasRelatedWork W1975040420 @default.
• W4225388925 hasRelatedWork W2019526079 @default.
• W4225388925 hasRelatedWork W2039140981 @default.
• W4225388925 hasRelatedWork W2061872883 @default.
• W4225388925 hasRelatedWork W2072214732 @default.
• W4225388925 hasRelatedWork W2077907491 @default.
• W4225388925 hasRelatedWork W2086839033 @default.
• W4225388925 hasRelatedWork W2312237506 @default.
• W4225388925 hasRelatedWork W2379458137 @default.
• W4225388925 hasRelatedWork W2392281067 @default.
• W4225388925 hasVolume "305" @default.
• W4225388925 isParatext "false" @default.
• W4225388925 isRetracted "false" @default.
• W4225388925 workType "article" @default.