Matches in SemOpenAlex for { <https://semopenalex.org/work/W4225407837> ?p ?o ?g. }
Showing items 1 to 88 of
88
with 100 items per page.
- W4225407837 abstract "Hypokalemia is a common electrolyte disturbance frequently encountered in clinical medicine. Animals with hypokalemia had a significant arginine vasopressin (AVP)-resistant polyuria with reduced expression of aquaporin 2 (AQP2) levels in the collecting ducts of the kidneys. The precise molecular mechanisms of hypokalemia-induced nephrogenic diabetes insipidus remain unresolved. AVP induced a marked increase in histone H3K27 acetylation (H3K27ac) across the body of the AQP2 gene, in the promoter and in upstream of the AQP2 transcriptional start site in mouse mpkCCD cell line (PMID: 29572403). The purpose of the present study was to investigate whether enhanced histone acetylation prevented decreased AQP2 expression and improved urinary concentrating defect in rats with hypokalemia. Male Wistar rats were fed potassium-free diet with or without 4-phenylbutyric acid (4-PBA) or a selective inhibitor of histone deacetylase 3 (HDAC3) RGFP966 for 4 days. 4-PBA or RGFP966 markedly increased AQP2 mRNA and protein levels in kidney inner medulla of rats with hypokalemia, which was associated with decreased urine output and increased urinary osmolality. Protein expression of H3K27ac was markedly decreased in kidney inner medulla of hypokalemic rats, which was significantly prevented by 4-PBA or RGFP966. Expression of H3K27ac was decreased in primary cultured inner medullary collecting duct cells (IMCD) or mpkCCD cells cultured in potassium-deprivation media, in particular, H3K27ac in the promoter region of AQP2 was markedly decreased, which was associated with reduced mRNA levels of AQP2. Protein expression of HDAC3 was significantly upregulated in both mpkCCD and IMCD cells in response to potassium deprivation; the binding of HDAC3 to the promoter of AQP2 was also dramatically increased. RGFP966 significantly increased expression of H3K27ac and AQP2 protein and enhanced the binding of H3K27ac to AQP2 in mpkCCD cells. In conclusion, HDAC3 inhibition prevented downregulation of AQP2 and improved urinary concentrating defect induced by potassium deprivation, likely through enhancing acetylation of H3K27 and transcription of AQP2." @default.
- W4225407837 created "2022-05-05" @default.
- W4225407837 creator A5015906121 @default.
- W4225407837 creator A5047894137 @default.
- W4225407837 creator A5051529232 @default.
- W4225407837 creator A5056118454 @default.
- W4225407837 creator A5057930159 @default.
- W4225407837 creator A5070690132 @default.
- W4225407837 creator A5071615222 @default.
- W4225407837 creator A5075124603 @default.
- W4225407837 creator A5087048206 @default.
- W4225407837 date "2022-05-01" @default.
- W4225407837 modified "2023-10-14" @default.
- W4225407837 title "HDAC3 Inhibition Improved Urinary Concentrating Defect in Hypokalemia by Promoting AQP2 Transcription" @default.
- W4225407837 doi "https://doi.org/10.1096/fasebj.2022.36.s1.r2825" @default.
- W4225407837 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35560517" @default.
- W4225407837 hasPublicationYear "2022" @default.
- W4225407837 type Work @default.
- W4225407837 citedByCount "0" @default.
- W4225407837 crossrefType "journal-article" @default.
- W4225407837 hasAuthorship W4225407837A5015906121 @default.
- W4225407837 hasAuthorship W4225407837A5047894137 @default.
- W4225407837 hasAuthorship W4225407837A5051529232 @default.
- W4225407837 hasAuthorship W4225407837A5056118454 @default.
- W4225407837 hasAuthorship W4225407837A5057930159 @default.
- W4225407837 hasAuthorship W4225407837A5070690132 @default.
- W4225407837 hasAuthorship W4225407837A5071615222 @default.
- W4225407837 hasAuthorship W4225407837A5075124603 @default.
- W4225407837 hasAuthorship W4225407837A5087048206 @default.
- W4225407837 hasConcept C104317684 @default.
- W4225407837 hasConcept C126322002 @default.
- W4225407837 hasConcept C127413603 @default.
- W4225407837 hasConcept C134018914 @default.
- W4225407837 hasConcept C185592680 @default.
- W4225407837 hasConcept C201289731 @default.
- W4225407837 hasConcept C2775845273 @default.
- W4225407837 hasConcept C2776370428 @default.
- W4225407837 hasConcept C2776958162 @default.
- W4225407837 hasConcept C2777464133 @default.
- W4225407837 hasConcept C2778242168 @default.
- W4225407837 hasConcept C2778305200 @default.
- W4225407837 hasConcept C2780091579 @default.
- W4225407837 hasConcept C55493867 @default.
- W4225407837 hasConcept C64927066 @default.
- W4225407837 hasConcept C71924100 @default.
- W4225407837 hasConcept C78519656 @default.
- W4225407837 hasConcept C85562759 @default.
- W4225407837 hasConcept C86803240 @default.
- W4225407837 hasConceptScore W4225407837C104317684 @default.
- W4225407837 hasConceptScore W4225407837C126322002 @default.
- W4225407837 hasConceptScore W4225407837C127413603 @default.
- W4225407837 hasConceptScore W4225407837C134018914 @default.
- W4225407837 hasConceptScore W4225407837C185592680 @default.
- W4225407837 hasConceptScore W4225407837C201289731 @default.
- W4225407837 hasConceptScore W4225407837C2775845273 @default.
- W4225407837 hasConceptScore W4225407837C2776370428 @default.
- W4225407837 hasConceptScore W4225407837C2776958162 @default.
- W4225407837 hasConceptScore W4225407837C2777464133 @default.
- W4225407837 hasConceptScore W4225407837C2778242168 @default.
- W4225407837 hasConceptScore W4225407837C2778305200 @default.
- W4225407837 hasConceptScore W4225407837C2780091579 @default.
- W4225407837 hasConceptScore W4225407837C55493867 @default.
- W4225407837 hasConceptScore W4225407837C64927066 @default.
- W4225407837 hasConceptScore W4225407837C71924100 @default.
- W4225407837 hasConceptScore W4225407837C78519656 @default.
- W4225407837 hasConceptScore W4225407837C85562759 @default.
- W4225407837 hasConceptScore W4225407837C86803240 @default.
- W4225407837 hasFunder F4320321001 @default.
- W4225407837 hasFunder F4320321921 @default.
- W4225407837 hasIssue "S1" @default.
- W4225407837 hasLocation W42254078371 @default.
- W4225407837 hasLocation W42254078372 @default.
- W4225407837 hasOpenAccess W4225407837 @default.
- W4225407837 hasPrimaryLocation W42254078371 @default.
- W4225407837 hasRelatedWork W1979292275 @default.
- W4225407837 hasRelatedWork W1993555913 @default.
- W4225407837 hasRelatedWork W2033746374 @default.
- W4225407837 hasRelatedWork W2078493988 @default.
- W4225407837 hasRelatedWork W2093712015 @default.
- W4225407837 hasRelatedWork W2107172074 @default.
- W4225407837 hasRelatedWork W2107814524 @default.
- W4225407837 hasRelatedWork W2182139610 @default.
- W4225407837 hasRelatedWork W2333895078 @default.
- W4225407837 hasRelatedWork W4225407837 @default.
- W4225407837 hasVolume "36" @default.
- W4225407837 isParatext "false" @default.
- W4225407837 isRetracted "false" @default.
- W4225407837 workType "article" @default.