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- W4225492790 endingPage "713" @default.
- W4225492790 startingPage "713" @default.
- W4225492790 abstract "Immunoglobulin G (IgG), a type of antibody, represents approximately 75% of serum antibodies in humans, and is the most common type of antibody found in blood circulation. Consequently, the development of simple, fast and reliable systems for IgG detection, which can be achieved using electrochemical sandwich-type immunosensors, is of considerable interest. In this study we have developed an immunosensor for human (H)-IgG using an inexpensive and very simple fabrication method based on ZnO nanorods (NRs) obtained through the electrodeposition of ZnO. The ZnO NRs were treated by electrodepositing a layer of reduced graphene oxide (rGO) to ensure an easy immobilization of the antibodies. On Indium Tin Oxide supported on Polyethylene Terephthalate/ZnO NRs/rGO substrate, the sandwich configuration of the immunosensor was built through different incubation steps, which were all optimized. The immunosensor is electrochemically active thanks to the presence of gold nanoparticles tagging the secondary antibody. The immunosensor was used to measure the current density of the hydrogen development reaction which is indirectly linked to the concentration of H-IgG. In this way the calibration curve was constructed obtaining a logarithmic linear range of 10-1000 ng/mL with a detection limit of few ng/mL and good sensitivity." @default.
- W4225492790 created "2022-05-05" @default.
- W4225492790 creator A5003063246 @default.
- W4225492790 creator A5006785124 @default.
- W4225492790 creator A5030886399 @default.
- W4225492790 creator A5033894652 @default.
- W4225492790 creator A5044819282 @default.
- W4225492790 creator A5062951393 @default.
- W4225492790 creator A5063229486 @default.
- W4225492790 creator A5085031055 @default.
- W4225492790 creator A5088694422 @default.
- W4225492790 date "2022-01-18" @default.
- W4225492790 modified "2023-10-17" @default.
- W4225492790 title "Electrochemical Synthesis of Zinc Oxide Nanostructures on Flexible Substrate and Application as an Electrochemical Immunoglobulin-G Immunosensor" @default.
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