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- W4225517140 abstract "Sinoatrial node (SAN) cells are the pacemakers of the heart. This study describes a method for culturing and infection of adult mouse SAN cells with FRET-based biosensors that can be exploited to examine signaling events. SAN cells cultured in media with blebbistatin or (S)-nitro-blebbistatin retain their morphology, protein distribution, action potential (AP) waveform, and cAMP dynamics for at least 40 h. SAN cells expressing targeted cAMP sensors show distinct β-adrenergic-mediated cAMP pools. Cyclic GMP, protein kinase A, Ca2+/CaM kinase II, and protein kinase D in SAN cells also show unique dynamics to different stimuli. Heart failure SAN cells show a decrease in cAMP and cGMP levels. In summary, a reliable method for maintaining adult mouse SAN cells in culture is presented, which facilitates studies of signaling networks and regulatory mechanisms during physiological and pathological conditions." @default.
- W4225517140 created "2022-05-05" @default.
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- W4225517140 date "2022-01-01" @default.
- W4225517140 modified "2023-10-14" @default.
- W4225517140 title "Deciphering cellular signals in adult mouse sinoatrial node cells" @default.
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- W4225517140 doi "https://doi.org/10.1016/j.isci.2021.103693" @default.
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