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- W4225519503 abstract "Abstract Background: Bacillus is a ubiquitous microorganism and is one of the most commercially important species widely used in industry, agriculture and healthcare. Bacillus is relatively well understood at the single-cell level; however, molecular tools that determine diversity and ecology of Bacillus community are limited, which limits our understanding of how the Bacillus community works. Results: In this study, we investigated the potential of the housekeeping gene gyrA as a molecular marker for determining the diversity of Bacillus species, then designed a new primer pair that specifically target gyrA ( gyrA 3). The rate of variation of the gyrA gene was higher than 16S rRNA gene in inter- and intraspecific Bacillus . And the amplification efficiency for Bacillus species diversity could be greatly improved by primer design, the novel primer pair gyrA 3 that can detect at least 92 Bacillus species and related species and showed its advantage in mock Bacillus community compared to 16S rRNA gene. For Bacillus amyloliquefaciens , Bacillus pumilus, and Bacillus megaterium , the high variability of the gyrA gene allows for more detailed clustering at the subspecies level that cannot be achieved by the 16S rRNA gene. Conclusion: Collectively, the gyrA gene provides better phylogenetic resolution than 16S rRNA gene and informs on the diversity of the Bacillus community, therefore gyrA gene may have broad application prospects in the study of Bacillus ecology." @default.
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- W4225519503 date "2022-04-20" @default.
- W4225519503 modified "2023-10-18" @default.
- W4225519503 title "Housekeeping gene gyrA, a potential molecular marker for Bacillus ecology study" @default.
- W4225519503 doi "https://doi.org/10.21203/rs.3.rs-1557860/v1" @default.
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