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• W4225522918 abstract "Most of the environmental stresses damage crops provoking economic losses. Molecular characterization of genotypes identifying suitable genes helps breeders to develop abiotic stress-tolerant crops. Among the omics technologies, transcriptomics represents the most informative techniques for global gene expression profiling of an organism, organ/tissue, or even cell at a given snapshot in time. In this context the next-generation sequencing technologies revolutionized plant transcriptomics, particularly nonmodel plants of economic interest, enhancing the high-throughput data generated from biological samples. This chapter shows an updated RNA-Seq (sequencing of RNA) overview covering scientific reports published in the last 5 years. The assortment of the data-mined RNA-Seq studies concerning relevant issues identified the most studied crops, abiotic stresses, plant organs/tissues, stress-treatment exposition time, global or specific gene expression profiling, noncoding RNA approach, extra omics data, supplementary physiological data, studied accession(s), transcriptome assembly approach, gene expression validation process, relative quantification method, and reference gene(s) in the qPCR assays. Besides, an overall RNA-Seq analysis workflow, covering data generation, raw data processing, and data analysis, was provided, together with some information involving transcriptome assembly, quality of the transcriptome assembly, transcript quantification, differential expression analysis, and annotation/functional analysis. Finally, in the functional genomics topic, the integration of multiomic data stands out, improving our understanding of genetic networks, which helps us to envision machine learning strategies and genome editing applications involving agronomic characteristics. These approaches may help plant breeders identify new genes and related metabolic subpathways associated with relevant agronomic traits." @default.
• W4225522918 created "2022-05-05" @default.
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• W4225522918 date "2022-01-01" @default.
• W4225522918 modified "2023-09-30" @default.
• W4225522918 title "Explore the RNA-sequencing and the next-generation sequencing in crops responding to abiotic stress" @default.
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• W4225522918 doi "https://doi.org/10.1016/b978-0-323-89778-5.00005-2" @default.
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