Matches in SemOpenAlex for { <https://semopenalex.org/work/W4225528146> ?p ?o ?g. }
Showing items 1 to 66 of
66
with 100 items per page.
- W4225528146 abstract "Abstract Background: Dual specificity phosphatase 7 (DUSP7) is a protein belonging to a broad group of phosphatases that can dephosphorylate phosphoserine/phosphothreonine as well as phosphotyrosine residues within the same substrate. DUSP7 has been linked to the negative regulation of mitogen activated protein kinases (MAPK), and in particular to the regulation of extracellular signal-regulated kinases 1 and 2 (ERK1/2). MAPKs play an important role in embryonic development, where their duration, magnitude, and spatiotemporal activity must be strictly controlled by other proteins, among others by DUSPs.Methods: In this study, we focused on the effect of DUSP7 depletion on the in vitro differentiation of mouse embryonic stem (ES) cells. Undifferentiated stem cells as well as cells at various days of in vitro differentiation protocol were used for analysis using mainly qPCR, western blotting and immunohistochemistry. Data are expressed as mean ± standard deviation (SD). Statistical analysis was assessed by t-test and by one- or two-way ANOVA, and by Bonferroni’s Multiple Comparison post hoc test. Values of P < 0.05 were considered to be statistically significant (∗ p < 0.05).Results: We showed that even though DUSP7 knock-out ES cells do retain some of their basic characteristics, when it comes to differentiation, they preferentially differentiate towards neural cells, while the formation of early cardiac mesoderm is repressed.Conclusions: Our data indicate that DUSP7 is necessary for the correct formation of neuroectoderm and cardiac mesoderm during the in vitro differentiation of ES cells." @default.
- W4225528146 created "2022-05-05" @default.
- W4225528146 creator A5029000240 @default.
- W4225528146 creator A5031162041 @default.
- W4225528146 creator A5055333268 @default.
- W4225528146 creator A5057590375 @default.
- W4225528146 creator A5065607808 @default.
- W4225528146 creator A5087317439 @default.
- W4225528146 date "2022-02-09" @default.
- W4225528146 modified "2023-10-17" @default.
- W4225528146 title "Dual Specificity Phosphatase 7 Drives the Formation of Cardiac Mesoderm in Mouse Embryonic Stem Cells" @default.
- W4225528146 doi "https://doi.org/10.21203/rs.3.rs-1324854/v1" @default.
- W4225528146 hasPublicationYear "2022" @default.
- W4225528146 type Work @default.
- W4225528146 citedByCount "0" @default.
- W4225528146 crossrefType "posted-content" @default.
- W4225528146 hasAuthorship W4225528146A5029000240 @default.
- W4225528146 hasAuthorship W4225528146A5031162041 @default.
- W4225528146 hasAuthorship W4225528146A5055333268 @default.
- W4225528146 hasAuthorship W4225528146A5057590375 @default.
- W4225528146 hasAuthorship W4225528146A5065607808 @default.
- W4225528146 hasAuthorship W4225528146A5087317439 @default.
- W4225528146 hasBestOaLocation W42255281461 @default.
- W4225528146 hasConcept C104317684 @default.
- W4225528146 hasConcept C11960822 @default.
- W4225528146 hasConcept C145103041 @default.
- W4225528146 hasConcept C148738053 @default.
- W4225528146 hasConcept C153911025 @default.
- W4225528146 hasConcept C178666793 @default.
- W4225528146 hasConcept C184235292 @default.
- W4225528146 hasConcept C2780915607 @default.
- W4225528146 hasConcept C28328180 @default.
- W4225528146 hasConcept C55493867 @default.
- W4225528146 hasConcept C57074206 @default.
- W4225528146 hasConcept C86803240 @default.
- W4225528146 hasConcept C95444343 @default.
- W4225528146 hasConceptScore W4225528146C104317684 @default.
- W4225528146 hasConceptScore W4225528146C11960822 @default.
- W4225528146 hasConceptScore W4225528146C145103041 @default.
- W4225528146 hasConceptScore W4225528146C148738053 @default.
- W4225528146 hasConceptScore W4225528146C153911025 @default.
- W4225528146 hasConceptScore W4225528146C178666793 @default.
- W4225528146 hasConceptScore W4225528146C184235292 @default.
- W4225528146 hasConceptScore W4225528146C2780915607 @default.
- W4225528146 hasConceptScore W4225528146C28328180 @default.
- W4225528146 hasConceptScore W4225528146C55493867 @default.
- W4225528146 hasConceptScore W4225528146C57074206 @default.
- W4225528146 hasConceptScore W4225528146C86803240 @default.
- W4225528146 hasConceptScore W4225528146C95444343 @default.
- W4225528146 hasLocation W42255281461 @default.
- W4225528146 hasLocation W42255281462 @default.
- W4225528146 hasOpenAccess W4225528146 @default.
- W4225528146 hasPrimaryLocation W42255281461 @default.
- W4225528146 hasRelatedWork W1920840680 @default.
- W4225528146 hasRelatedWork W1973133102 @default.
- W4225528146 hasRelatedWork W1991163038 @default.
- W4225528146 hasRelatedWork W2009519731 @default.
- W4225528146 hasRelatedWork W2015725807 @default.
- W4225528146 hasRelatedWork W2063399102 @default.
- W4225528146 hasRelatedWork W2081246372 @default.
- W4225528146 hasRelatedWork W2091587849 @default.
- W4225528146 hasRelatedWork W2105795952 @default.
- W4225528146 hasRelatedWork W3140155232 @default.
- W4225528146 isParatext "false" @default.
- W4225528146 isRetracted "false" @default.
- W4225528146 workType "article" @default.