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- W4225540246 abstract "Integrin adhesion complexes (IACs) are integrin-based plasma-membrane-associated compartments where cells sense environmental cues. The physical mechanisms and molecular interactions that mediate initial IAC formation are unclear. We found that both p130Cas ('Cas') and Focal adhesion kinase ('FAK') undergo liquid-liquid phase separation in vitro under physiologic conditions. Cas- and FAK- driven phase separation is sufficient to reconstitute kindlin-dependent integrin clustering in vitro with recombinant mammalian proteins. In vitro condensates and IACs in mouse embryonic fibroblasts (MEFs) exhibit similar sensitivities to environmental perturbations including changes in temperature and pH. Furthermore, mutations that inhibit or enhance phase separation in vitro reduce or increase the number of IACs in MEFs, respectively. Finally, we find that the Cas and FAK pathways act synergistically to promote phase separation, integrin clustering, IAC formation and partitioning of key components in vitro and in cells. We propose that Cas- and FAK-driven phase separation provides an intracellular trigger for integrin clustering and nascent IAC formation." @default.
- W4225540246 created "2022-05-05" @default.
- W4225540246 creator A5003361057 @default.
- W4225540246 creator A5012173770 @default.
- W4225540246 creator A5048073198 @default.
- W4225540246 creator A5058093328 @default.
- W4225540246 date "2022-01-20" @default.
- W4225540246 modified "2023-10-04" @default.
- W4225540246 title "Synergistic phase separation of two pathways promotes integrin clustering and nascent adhesion formation" @default.
- W4225540246 cites W1576773659 @default.
- W4225540246 cites W1811064827 @default.
- W4225540246 cites W1838945435 @default.
- W4225540246 cites W1854281301 @default.
- W4225540246 cites W1936657395 @default.
- W4225540246 cites W1964211098 @default.
- W4225540246 cites W1975537850 @default.
- W4225540246 cites W1977585994 @default.
- W4225540246 cites W1979038578 @default.
- W4225540246 cites W1981527414 @default.
- W4225540246 cites W1988194926 @default.
- W4225540246 cites W1988593586 @default.
- W4225540246 cites W1989197126 @default.
- W4225540246 cites W1989718733 @default.
- W4225540246 cites W1994825452 @default.
- W4225540246 cites W2000638372 @default.
- W4225540246 cites W2015341098 @default.
- W4225540246 cites W2021027041 @default.
- W4225540246 cites W2022350385 @default.
- W4225540246 cites W2026306905 @default.
- W4225540246 cites W2027265162 @default.
- W4225540246 cites W2028116568 @default.
- W4225540246 cites W2029561530 @default.
- W4225540246 cites W2031183440 @default.
- W4225540246 cites W2031261181 @default.
- W4225540246 cites W2031339765 @default.
- W4225540246 cites W2034716886 @default.
- W4225540246 cites W2049718632 @default.
- W4225540246 cites W2052164164 @default.
- W4225540246 cites W2053001031 @default.
- W4225540246 cites W2054172043 @default.
- W4225540246 cites W2055334948 @default.
- W4225540246 cites W2059492700 @default.
- W4225540246 cites W2060637453 @default.
- W4225540246 cites W2084302112 @default.
- W4225540246 cites W2084531881 @default.
- W4225540246 cites W2085767196 @default.
- W4225540246 cites W2085978470 @default.
- W4225540246 cites W2086636087 @default.
- W4225540246 cites W2096658419 @default.
- W4225540246 cites W2101480539 @default.
- W4225540246 cites W2112630221 @default.
- W4225540246 cites W2112924183 @default.
- W4225540246 cites W2113263416 @default.
- W4225540246 cites W2113338297 @default.
- W4225540246 cites W2114975607 @default.
- W4225540246 cites W2116045343 @default.
- W4225540246 cites W2116431176 @default.
- W4225540246 cites W2118585187 @default.
- W4225540246 cites W2125272201 @default.
- W4225540246 cites W2125292721 @default.
- W4225540246 cites W2128237450 @default.
- W4225540246 cites W2132488847 @default.
- W4225540246 cites W2132830901 @default.
- W4225540246 cites W2134338037 @default.
- W4225540246 cites W2143920361 @default.
- W4225540246 cites W2145392559 @default.
- W4225540246 cites W2149532912 @default.
- W4225540246 cites W2150020603 @default.
- W4225540246 cites W2150338395 @default.
- W4225540246 cites W2159119531 @default.
- W4225540246 cites W2159425769 @default.
- W4225540246 cites W2160518415 @default.
- W4225540246 cites W2164160141 @default.
- W4225540246 cites W2168639701 @default.
- W4225540246 cites W2171957474 @default.
- W4225540246 cites W2172098255 @default.
- W4225540246 cites W2175966865 @default.
- W4225540246 cites W2211191608 @default.
- W4225540246 cites W2277501221 @default.
- W4225540246 cites W2292845959 @default.
- W4225540246 cites W2335345046 @default.
- W4225540246 cites W2342193534 @default.
- W4225540246 cites W2402791934 @default.
- W4225540246 cites W2431220377 @default.
- W4225540246 cites W2461977200 @default.
- W4225540246 cites W2468082290 @default.
- W4225540246 cites W2509111410 @default.
- W4225540246 cites W2590568317 @default.
- W4225540246 cites W2592563316 @default.
- W4225540246 cites W2594954404 @default.
- W4225540246 cites W2738571822 @default.
- W4225540246 cites W2750726194 @default.
- W4225540246 cites W2753484764 @default.
- W4225540246 cites W2753960063 @default.
- W4225540246 cites W2756821926 @default.
- W4225540246 cites W2784370070 @default.
- W4225540246 cites W2784401093 @default.
- W4225540246 cites W2786469974 @default.
- W4225540246 cites W2801356113 @default.
- W4225540246 cites W2887994055 @default.