FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4225564607> ?p ?o ?g. }

• W4225564607 abstract "At present, oral antidepressants are commonly used in the clinical treatment of depression. However, the current drug treatment may lead to more serious adverse reactions. Therefore, we focus on Chinese traditional medicine, trying to find an effective and safe alternative or complementary medicine. Crocus sativus (saffron) is a traditional Chinese herbal medicine, which is typically used in the clinic to regulate anxiety, insomnia, amnesia, and other mental disorder. The study aimed to explore the neuroprotective effect of ethanol extract of saffron (EES) on corticosterone (CORT)- induced injury in PC12 cells and further explored its potential mechanism.The authenticity of saffron and the active components of EES were identified by a water test and ultra-performance liquid chromatography-time of flight mass spectrometry system. The screening of cytotoxicity for PC12 cells was incubated with EES in different concentrations for 24 h, and the protective efficacy of EES on CORT (500 μM) -induced PC12 cell injury, cell viability was assessed by Cell Counting Kit-8 (CCK-8) assay. The differentially expressed genes (DEGs) of EES-protected PC12 cells were analyzed using the RNA-seq method, and the results were analyzed for GO and KEGG enrichment. The results of RNA-seq were verified by qPCR analysis.The saffron was initially identified as authentic in the water test and 10 compounds were identified by Ultra Performance Liquid Chromatography (UPLC)- Mass Spectrometry (MS). The results of CCK-8 demonstrated that EES at concentrations above 640 μg/mL exerted a certain cytotoxic effect, and PC12 cells pretreated with EES (20, 40, and 80 μg/mL) significantly reversed the 500 μM CORT-induced cell death. RNA-seq analysis showed that EES regulated 246 differential genes, which were mainly enriched in the MAPK signaling pathway. Dusp5, Dusp6, Gadd45b, Gadd45G, and Pdgfc were further validated by qPCR. Experimental data showed that the results of qPCR were consistent with RNA-seq.These findings provide an innovative understanding of the molecular mechanism of the protective effect of EES on PC12 cells at the molecular transcription level, and Dusp5, Dusp6, Gadd45b, Gadd45g, and Pdgfc may be potential novel targets for antidepressant treatment." @default.
• W4225564607 created "2022-05-05" @default.
• W4225564607 creator A5008171524 @default.
• W4225564607 creator A5031646192 @default.
• W4225564607 creator A5049616921 @default.
• W4225564607 creator A5075357869 @default.
• W4225564607 date "2022-01-31" @default.
• W4225564607 modified "2023-10-09" @default.
• W4225564607 title "RNA-seq based transcriptome analysis of ethanol extract of saffron protective effect against corticosterone-induced PC12 cell injury" @default.
• W4225564607 cites W114637156 @default.
• W4225564607 cites W1200555839 @default.
• W4225564607 cites W1483881410 @default.
• W4225564607 cites W1579024202 @default.
• W4225564607 cites W167747296 @default.
• W4225564607 cites W1965790167 @default.
• W4225564607 cites W1981509058 @default.
• W4225564607 cites W1984615306 @default.
• W4225564607 cites W1984708728 @default.
• W4225564607 cites W1994044160 @default.
• W4225564607 cites W1998846962 @default.
• W4225564607 cites W1998907224 @default.
• W4225564607 cites W1999360913 @default.
• W4225564607 cites W2000644842 @default.
• W4225564607 cites W2002553836 @default.
• W4225564607 cites W2035229071 @default.
• W4225564607 cites W2060035717 @default.
• W4225564607 cites W2062289724 @default.
• W4225564607 cites W2064723004 @default.
• W4225564607 cites W2092075716 @default.
• W4225564607 cites W2117236226 @default.
• W4225564607 cites W2133137182 @default.
• W4225564607 cites W2153122353 @default.
• W4225564607 cites W2167898988 @default.
• W4225564607 cites W2324138891 @default.
• W4225564607 cites W2489075680 @default.
• W4225564607 cites W2519531315 @default.
• W4225564607 cites W2586384568 @default.
• W4225564607 cites W2627088574 @default.
• W4225564607 cites W2791245151 @default.
• W4225564607 cites W2805724135 @default.
• W4225564607 cites W2900825389 @default.
• W4225564607 cites W2928665623 @default.
• W4225564607 cites W2977695626 @default.
• W4225564607 cites W3042630400 @default.
• W4225564607 cites W3190259404 @default.
• W4225564607 cites W4211263234 @default.
• W4225564607 doi "https://doi.org/10.1186/s12906-022-03516-1" @default.
• W4225564607 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35101009" @default.
• W4225564607 hasPublicationYear "2022" @default.
• W4225564607 type Work @default.
• W4225564607 citedByCount "3" @default.
• W4225564607 countsByYear W42255646072022 @default.
• W4225564607 countsByYear W42255646072023 @default.
• W4225564607 crossrefType "journal-article" @default.
• W4225564607 hasAuthorship W4225564607A5008171524 @default.
• W4225564607 hasAuthorship W4225564607A5031646192 @default.
• W4225564607 hasAuthorship W4225564607A5049616921 @default.
• W4225564607 hasAuthorship W4225564607A5075357869 @default.
• W4225564607 hasBestOaLocation W42255646071 @default.
• W4225564607 hasConcept C104317684 @default.
• W4225564607 hasConcept C142724271 @default.
• W4225564607 hasConcept C150194340 @default.
• W4225564607 hasConcept C152724338 @default.
• W4225564607 hasConcept C162317418 @default.
• W4225564607 hasConcept C185592680 @default.
• W4225564607 hasConcept C188947578 @default.
• W4225564607 hasConcept C204787440 @default.
• W4225564607 hasConcept C25498285 @default.
• W4225564607 hasConcept C2779571273 @default.
• W4225564607 hasConcept C2780352252 @default.
• W4225564607 hasConcept C55493867 @default.
• W4225564607 hasConcept C556039675 @default.
• W4225564607 hasConcept C71315377 @default.
• W4225564607 hasConcept C71924100 @default.
• W4225564607 hasConcept C98274493 @default.
• W4225564607 hasConceptScore W4225564607C104317684 @default.
• W4225564607 hasConceptScore W4225564607C142724271 @default.
• W4225564607 hasConceptScore W4225564607C150194340 @default.
• W4225564607 hasConceptScore W4225564607C152724338 @default.
• W4225564607 hasConceptScore W4225564607C162317418 @default.
• W4225564607 hasConceptScore W4225564607C185592680 @default.
• W4225564607 hasConceptScore W4225564607C188947578 @default.
• W4225564607 hasConceptScore W4225564607C204787440 @default.
• W4225564607 hasConceptScore W4225564607C25498285 @default.
• W4225564607 hasConceptScore W4225564607C2779571273 @default.
• W4225564607 hasConceptScore W4225564607C2780352252 @default.
• W4225564607 hasConceptScore W4225564607C55493867 @default.
• W4225564607 hasConceptScore W4225564607C556039675 @default.
• W4225564607 hasConceptScore W4225564607C71315377 @default.
• W4225564607 hasConceptScore W4225564607C71924100 @default.
• W4225564607 hasConceptScore W4225564607C98274493 @default.
• W4225564607 hasIssue "1" @default.
• W4225564607 hasLocation W42255646071 @default.
• W4225564607 hasLocation W42255646072 @default.
• W4225564607 hasLocation W42255646073 @default.
• W4225564607 hasLocation W42255646074 @default.
• W4225564607 hasOpenAccess W4225564607 @default.
• W4225564607 hasPrimaryLocation W42255646071 @default.
• W4225564607 hasRelatedWork W1556453709 @default.
• W4225564607 hasRelatedWork W2011754050 @default.

• next