Matches in SemOpenAlex for { <https://semopenalex.org/work/W4225566817> ?p ?o ?g. }
- W4225566817 endingPage "107463" @default.
- W4225566817 startingPage "107463" @default.
- W4225566817 abstract "A novel electrochemical immunoassay based on nanobody heptamer for ultrasensitive detection of alpha fetoprotein (AFP) is proposed. First, AFP heptamer fusion protein (A1-C4bp α) is prepared via fusing the AFP-specific nanobody (A1) with the C-terminal fragment of C4-binding protein (C4bp α). Then A1-C4bp α heptamer with higher affinity is used as the molecule recognizing probe to capture numerous the AFP, increasing the sensitivity of the immunosensor. Moreover, AuNPs decorated zeolitic imidazolate framework ([email protected]) with large surface area, and abundant binding sites serves an ideal nanocarrier to increase heptamer loading. Multiwalled carbon nanotube (MWCNTs) with excellent electrical conductivity was immobilized on glassy carbon electrode (GCE) to enhance electrochemical signals. The coupling between the [email protected]@MWCNTs and heptamer technologies constructed an ultrasensitive and specific immunosensor for AFP detection in the range of 0.1 to 105 pg mL−1 with a lower detection limit of 0.033 pg mL−1. The as-prepared electrochemical sensing exhibited excellent performance and could be employed to determine AFP with satisfactory results in actual serum samples, suggesting that the proposed strategy for nanobody multimerization provides an alternative scheme for enhancing sensitivity in important biomarkers detection." @default.
- W4225566817 created "2022-05-05" @default.
- W4225566817 creator A5014708668 @default.
- W4225566817 creator A5014969873 @default.
- W4225566817 creator A5020559854 @default.
- W4225566817 creator A5021610484 @default.
- W4225566817 creator A5039412958 @default.
- W4225566817 creator A5064927953 @default.
- W4225566817 creator A5068352543 @default.
- W4225566817 creator A5081675173 @default.
- W4225566817 creator A5084727581 @default.
- W4225566817 date "2022-08-01" @default.
- W4225566817 modified "2023-10-17" @default.
- W4225566817 title "An innovative electrochemical immunosensor based on nanobody heptamer and AuNPs@ZIF-8 nanocomposites as support for the detection of alpha fetoprotein in serum" @default.
- W4225566817 cites W2008908847 @default.
- W4225566817 cites W2013031612 @default.
- W4225566817 cites W2057278101 @default.
- W4225566817 cites W2070824458 @default.
- W4225566817 cites W2101710429 @default.
- W4225566817 cites W2147636934 @default.
- W4225566817 cites W2164148151 @default.
- W4225566817 cites W2275428203 @default.
- W4225566817 cites W2319682162 @default.
- W4225566817 cites W2322925311 @default.
- W4225566817 cites W2343861500 @default.
- W4225566817 cites W2500547657 @default.
- W4225566817 cites W2644642324 @default.
- W4225566817 cites W2888272651 @default.
- W4225566817 cites W2899036147 @default.
- W4225566817 cites W2900183473 @default.
- W4225566817 cites W2912339349 @default.
- W4225566817 cites W2933595696 @default.
- W4225566817 cites W2937090481 @default.
- W4225566817 cites W2940560280 @default.
- W4225566817 cites W2948162403 @default.
- W4225566817 cites W2958131887 @default.
- W4225566817 cites W2970622165 @default.
- W4225566817 cites W2972565386 @default.
- W4225566817 cites W2986379465 @default.
- W4225566817 cites W2986851553 @default.
- W4225566817 cites W2990861768 @default.
- W4225566817 cites W2990942992 @default.
- W4225566817 cites W3001204754 @default.
- W4225566817 cites W3012906096 @default.
- W4225566817 cites W3015365726 @default.
- W4225566817 cites W3022322277 @default.
- W4225566817 cites W3036507685 @default.
- W4225566817 cites W3095970015 @default.
- W4225566817 cites W3119938485 @default.
- W4225566817 cites W3123006030 @default.
- W4225566817 cites W3129214233 @default.
- W4225566817 cites W3155749168 @default.
- W4225566817 cites W3158752642 @default.
- W4225566817 cites W3164799269 @default.
- W4225566817 cites W3166200508 @default.
- W4225566817 cites W3177184182 @default.
- W4225566817 cites W3192472718 @default.
- W4225566817 cites W4200102409 @default.
- W4225566817 doi "https://doi.org/10.1016/j.microc.2022.107463" @default.
- W4225566817 hasPublicationYear "2022" @default.
- W4225566817 type Work @default.
- W4225566817 citedByCount "9" @default.
- W4225566817 countsByYear W42255668172022 @default.
- W4225566817 countsByYear W42255668172023 @default.
- W4225566817 crossrefType "journal-article" @default.
- W4225566817 hasAuthorship W4225566817A5014708668 @default.
- W4225566817 hasAuthorship W4225566817A5014969873 @default.
- W4225566817 hasAuthorship W4225566817A5020559854 @default.
- W4225566817 hasAuthorship W4225566817A5021610484 @default.
- W4225566817 hasAuthorship W4225566817A5039412958 @default.
- W4225566817 hasAuthorship W4225566817A5064927953 @default.
- W4225566817 hasAuthorship W4225566817A5068352543 @default.
- W4225566817 hasAuthorship W4225566817A5081675173 @default.
- W4225566817 hasAuthorship W4225566817A5084727581 @default.
- W4225566817 hasConcept C10349863 @default.
- W4225566817 hasConcept C119128265 @default.
- W4225566817 hasConcept C147789679 @default.
- W4225566817 hasConcept C150394285 @default.
- W4225566817 hasConcept C159654299 @default.
- W4225566817 hasConcept C171250308 @default.
- W4225566817 hasConcept C17525397 @default.
- W4225566817 hasConcept C178790620 @default.
- W4225566817 hasConcept C179366358 @default.
- W4225566817 hasConcept C185592680 @default.
- W4225566817 hasConcept C192562407 @default.
- W4225566817 hasConcept C203014093 @default.
- W4225566817 hasConcept C21951064 @default.
- W4225566817 hasConcept C2780420688 @default.
- W4225566817 hasConcept C27983359 @default.
- W4225566817 hasConcept C43617362 @default.
- W4225566817 hasConcept C52859227 @default.
- W4225566817 hasConcept C86803240 @default.
- W4225566817 hasConceptScore W4225566817C10349863 @default.
- W4225566817 hasConceptScore W4225566817C119128265 @default.
- W4225566817 hasConceptScore W4225566817C147789679 @default.
- W4225566817 hasConceptScore W4225566817C150394285 @default.
- W4225566817 hasConceptScore W4225566817C159654299 @default.
- W4225566817 hasConceptScore W4225566817C171250308 @default.