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• W4225568512 abstract "Abstract Motivation Single-cell technologies play a crucial role in revolutionizing biological research over the past decade, which strengthens our understanding in cell differentiation, development and regulation from a single-cell level perspective. Single-cell RNA sequencing (scRNA-seq) is one of the most common single cell technologies, which enables probing transcriptional states in thousands of cells in one experiment. Identification of cell types from scRNA-seq measurements is a fundamental and crucial question to answer. Most previous studies directly take gene expression as input while ignoring the comprehensive gene–gene interactions. Results We propose scGraph, an automatic cell identification algorithm leveraging gene interaction relationships to enhance the performance of the cell-type identification. scGraph is based on a graph neural network to aggregate the information of interacting genes. In a series of experiments, we demonstrate that scGraph is accurate and outperforms eight comparison methods in the task of cell-type identification. Moreover, scGraph automatically learns the gene interaction relationships from biological data and the pathway enrichment analysis shows consistent findings with previous analysis, providing insights on the analysis of regulatory mechanism. Availability and implementation scGraph is freely available at https://github.com/QijinYin/scGraph and https://figshare.com/articles/software/scGraph/17157743. Supplementary information Supplementary data are available at Bioinformatics online." @default.
• W4225568512 created "2022-05-05" @default.
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• W4225568512 date "2022-04-08" @default.
• W4225568512 modified "2023-10-18" @default.
• W4225568512 title "scGraph: a graph neural network-based approach to automatically identify cell types" @default.
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• W4225568512 doi "https://doi.org/10.1093/bioinformatics/btac199" @default.
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