FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4225575505> ?p ?o ?g. }

• W4225575505 endingPage "112" @default.
• W4225575505 startingPage "101" @default.
• W4225575505 abstract "β-hydroxybutyric acid is the most sensitive indicator in ketoacidosis detection, and accounts for nearly 78% of the ketone bodies. Diaphorase is commonly used to detect the β-hydroxybutyric acid in clinical diagnosis. However, the extraction of diaphorase from animal myocardium is complex and low-yield, which is not convenient for large-scale production. In this study, a diaphorase from Geobacillus sp. Y4.1MC1 was efficiently heterologous expressed and purified in E. coli with a yield of 110 mg/L culture. The optimal temperature and pH of this recombinant diaphorase (rDIA) were 55 °C and 6.5, respectively. It was proved that rDIA was a dual acid- and thermo-stable enzyme, and which showed much more accurate detection of β-hydroxybutyric acid than the commercial enzyme. Additionally, we also investigated the molecular interaction of rDIA with the substrate, and the conformation transition in different pH values by using homology modeling and molecular dynamics simulation. The results showed that 141-161 domain of rDIA played important role in the structure changes and conformations transmission at different pH values. Moreover, it was predicted that F105W, F105R, and M186R mutants were able to improve the binding affinity of rDIA, and A2Y, P35F, Q36D, N210L, F211Y mutants were benefit for the stability of rDIA." @default.
• W4225575505 created "2022-05-05" @default.
• W4225575505 creator A5039252608 @default.
• W4225575505 creator A5052907339 @default.
• W4225575505 creator A5061402622 @default.
• W4225575505 creator A5090566557 @default.
• W4225575505 creator A5091400199 @default.
• W4225575505 date "2022-01-01" @default.
• W4225575505 modified "2023-09-26" @default.
• W4225575505 title "Heterologous expression, characterization and evolution prediction of a diaphorase from Geobacillus sp. Y4.1MC1" @default.
• W4225575505 cites W1482076568 @default.
• W4225575505 cites W1853063216 @default.
• W4225575505 cites W1965935659 @default.
• W4225575505 cites W1967281092 @default.
• W4225575505 cites W1970547520 @default.
• W4225575505 cites W1973280222 @default.
• W4225575505 cites W1975428856 @default.
• W4225575505 cites W1977926384 @default.
• W4225575505 cites W1984964811 @default.
• W4225575505 cites W1985248562 @default.
• W4225575505 cites W1987268093 @default.
• W4225575505 cites W1991728666 @default.
• W4225575505 cites W1997097990 @default.
• W4225575505 cites W2006913186 @default.
• W4225575505 cites W2008708467 @default.
• W4225575505 cites W2025787220 @default.
• W4225575505 cites W2029667189 @default.
• W4225575505 cites W2046276677 @default.
• W4225575505 cites W2050096343 @default.
• W4225575505 cites W2070312310 @default.
• W4225575505 cites W2071837299 @default.
• W4225575505 cites W2076154818 @default.
• W4225575505 cites W2122832248 @default.
• W4225575505 cites W2140237162 @default.
• W4225575505 cites W2144288821 @default.
• W4225575505 cites W2161893186 @default.
• W4225575505 cites W2528366847 @default.
• W4225575505 cites W2607592965 @default.
• W4225575505 cites W2624605523 @default.
• W4225575505 cites W2791834091 @default.
• W4225575505 cites W2804283711 @default.
• W4225575505 cites W3113228129 @default.
• W4225575505 cites W4212879092 @default.
• W4225575505 doi "https://doi.org/10.1007/s10529-021-03215-w" @default.
• W4225575505 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35001212" @default.
• W4225575505 hasPublicationYear "2022" @default.
• W4225575505 type Work @default.
• W4225575505 citedByCount "0" @default.
• W4225575505 crossrefType "journal-article" @default.
• W4225575505 hasAuthorship W4225575505A5039252608 @default.
• W4225575505 hasAuthorship W4225575505A5052907339 @default.
• W4225575505 hasAuthorship W4225575505A5061402622 @default.
• W4225575505 hasAuthorship W4225575505A5090566557 @default.
• W4225575505 hasAuthorship W4225575505A5091400199 @default.
• W4225575505 hasBestOaLocation W42255755052 @default.
• W4225575505 hasConcept C104317684 @default.
• W4225575505 hasConcept C108305142 @default.
• W4225575505 hasConcept C134121241 @default.
• W4225575505 hasConcept C143065580 @default.
• W4225575505 hasConcept C181199279 @default.
• W4225575505 hasConcept C185592680 @default.
• W4225575505 hasConcept C191897082 @default.
• W4225575505 hasConcept C192562407 @default.
• W4225575505 hasConcept C2780115692 @default.
• W4225575505 hasConcept C40767141 @default.
• W4225575505 hasConcept C55493867 @default.
• W4225575505 hasConcept C86803240 @default.
• W4225575505 hasConceptScore W4225575505C104317684 @default.
• W4225575505 hasConceptScore W4225575505C108305142 @default.
• W4225575505 hasConceptScore W4225575505C134121241 @default.
• W4225575505 hasConceptScore W4225575505C143065580 @default.
• W4225575505 hasConceptScore W4225575505C181199279 @default.
• W4225575505 hasConceptScore W4225575505C185592680 @default.
• W4225575505 hasConceptScore W4225575505C191897082 @default.
• W4225575505 hasConceptScore W4225575505C192562407 @default.
• W4225575505 hasConceptScore W4225575505C2780115692 @default.
• W4225575505 hasConceptScore W4225575505C40767141 @default.
• W4225575505 hasConceptScore W4225575505C55493867 @default.
• W4225575505 hasConceptScore W4225575505C86803240 @default.
• W4225575505 hasFunder F4320321543 @default.
• W4225575505 hasIssue "1" @default.
• W4225575505 hasLocation W42255755051 @default.
• W4225575505 hasLocation W42255755052 @default.
• W4225575505 hasLocation W42255755053 @default.
• W4225575505 hasOpenAccess W4225575505 @default.
• W4225575505 hasPrimaryLocation W42255755051 @default.
• W4225575505 hasRelatedWork W1482758805 @default.
• W4225575505 hasRelatedWork W2044140285 @default.
• W4225575505 hasRelatedWork W2051173107 @default.
• W4225575505 hasRelatedWork W2051990464 @default.
• W4225575505 hasRelatedWork W2073899828 @default.
• W4225575505 hasRelatedWork W2082277535 @default.
• W4225575505 hasRelatedWork W2361682150 @default.
• W4225575505 hasRelatedWork W2810523566 @default.
• W4225575505 hasRelatedWork W2885499617 @default.
• W4225575505 hasRelatedWork W4367366390 @default.
• W4225575505 hasVolume "44" @default.
• W4225575505 isParatext "false" @default.

• next