FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4225577948> ?p ?o ?g. }

• W4225577948 abstract "The importance of alveolar macrophages has been reported in many toxicology/immunology studies. Alveolar macrophages release interleukin (IL)-1α as a damage-associated molecular pattern (DAMP) when stimulated by fine particles. However, it is unclear whether cell isolation procedures affect ex vivo particle-induced responses in primary mouse alveolar macrophages (mAM). In this study, effects of injection buffer volume used to perform bronchoalveolar lavage fluid (BALF) collection to isolate mAM for use in ex vivo particle-induced responses were assessed. Among the mAM obtained from BALF collected using a 0.55 or 0.75 ml, but not a 1.0 ml buffer injection volume, decreased cell viability and IL-1α release were observed when cells were stimulated ex vivo with silica crystal or aluminum salt. Injected buffer composition did not affect the IL-1α release. On the other hand, IL-6 secretion induced by lipopolysaccharide (LPS) did not differ among mAM obtained from BALF collected using the different volumes. Expression levels of cell surface markers like CD11c, SiglecF, and CD64 did not differ among mAM obtained from BALF collected using the different injection buffer volumes. IL-1α release (and also necroptosis) induced by ex vivoparticle stimulation was suppressed by RIPK3 inhibitor or cytochalasin D co-treatment. Decreases in RIPK3 phosphorylation were noted in mAM obtained in BALF collected using the 1.0 ml injection volume compared with mAM obtained in BALF using 0.55 or 0.75 ml buffer. These observations illustrate that larger volumes of buffer used to collect BALF from mice can affect sensitivity of the isolated mAM to ex vivo particle-induced responses by inhibiting their functions." @default.
• W4225577948 created "2022-05-05" @default.
• W4225577948 creator A5029899367 @default.
• W4225577948 creator A5040425668 @default.
• W4225577948 creator A5058573964 @default.
• W4225577948 creator A5067391422 @default.
• W4225577948 creator A5068950132 @default.
• W4225577948 date "2021-01-01" @default.
• W4225577948 modified "2023-10-17" @default.
• W4225577948 title "Impact of injection buffer volume to perform bronchoalveolar lavage fluid collection for isolating alveolar macrophages to investigate fine particle-induced IL-1α secretion" @default.
• W4225577948 cites W1966547087 @default.
• W4225577948 cites W1976907957 @default.
• W4225577948 cites W1985859986 @default.
• W4225577948 cites W1997396254 @default.
• W4225577948 cites W2006776527 @default.
• W4225577948 cites W2008279380 @default.
• W4225577948 cites W2025134962 @default.
• W4225577948 cites W2042851093 @default.
• W4225577948 cites W2052853635 @default.
• W4225577948 cites W2061177902 @default.
• W4225577948 cites W2061600879 @default.
• W4225577948 cites W2063563210 @default.
• W4225577948 cites W2070024570 @default.
• W4225577948 cites W2070743437 @default.
• W4225577948 cites W2074213282 @default.
• W4225577948 cites W2078334493 @default.
• W4225577948 cites W2089836937 @default.
• W4225577948 cites W2101286028 @default.
• W4225577948 cites W2103160677 @default.
• W4225577948 cites W2104058942 @default.
• W4225577948 cites W2108934505 @default.
• W4225577948 cites W2111343777 @default.
• W4225577948 cites W2119188930 @default.
• W4225577948 cites W2120138008 @default.
• W4225577948 cites W2126384811 @default.
• W4225577948 cites W2134941921 @default.
• W4225577948 cites W2136722399 @default.
• W4225577948 cites W2145621175 @default.
• W4225577948 cites W2150107812 @default.
• W4225577948 cites W2166431583 @default.
• W4225577948 cites W2268619028 @default.
• W4225577948 cites W2276614885 @default.
• W4225577948 cites W2299138507 @default.
• W4225577948 cites W2563643476 @default.
• W4225577948 cites W2735726751 @default.
• W4225577948 cites W2765851264 @default.
• W4225577948 cites W2766532941 @default.
• W4225577948 cites W2767487209 @default.
• W4225577948 cites W2793171481 @default.
• W4225577948 cites W2804172422 @default.
• W4225577948 cites W2884180544 @default.
• W4225577948 cites W2922341654 @default.
• W4225577948 cites W2963522010 @default.
• W4225577948 cites W4237119264 @default.
• W4225577948 doi "https://doi.org/10.1080/1547691x.2021.1979699" @default.
• W4225577948 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34761701" @default.
• W4225577948 hasPublicationYear "2021" @default.
• W4225577948 type Work @default.
• W4225577948 citedByCount "0" @default.
• W4225577948 crossrefType "journal-article" @default.
• W4225577948 hasAuthorship W4225577948A5029899367 @default.
• W4225577948 hasAuthorship W4225577948A5040425668 @default.
• W4225577948 hasAuthorship W4225577948A5058573964 @default.
• W4225577948 hasAuthorship W4225577948A5067391422 @default.
• W4225577948 hasAuthorship W4225577948A5068950132 @default.
• W4225577948 hasBestOaLocation W42255779481 @default.
• W4225577948 hasConcept C104317684 @default.
• W4225577948 hasConcept C126322002 @default.
• W4225577948 hasConcept C127716648 @default.
• W4225577948 hasConcept C150903083 @default.
• W4225577948 hasConcept C153911025 @default.
• W4225577948 hasConcept C178790620 @default.
• W4225577948 hasConcept C185592680 @default.
• W4225577948 hasConcept C202751555 @default.
• W4225577948 hasConcept C203014093 @default.
• W4225577948 hasConcept C207001950 @default.
• W4225577948 hasConcept C26291073 @default.
• W4225577948 hasConcept C2777714996 @default.
• W4225577948 hasConcept C2777961210 @default.
• W4225577948 hasConcept C2778754761 @default.
• W4225577948 hasConcept C2778996431 @default.
• W4225577948 hasConcept C2779919695 @default.
• W4225577948 hasConcept C49039625 @default.
• W4225577948 hasConcept C55493867 @default.
• W4225577948 hasConcept C71924100 @default.
• W4225577948 hasConcept C86803240 @default.
• W4225577948 hasConceptScore W4225577948C104317684 @default.
• W4225577948 hasConceptScore W4225577948C126322002 @default.
• W4225577948 hasConceptScore W4225577948C127716648 @default.
• W4225577948 hasConceptScore W4225577948C150903083 @default.
• W4225577948 hasConceptScore W4225577948C153911025 @default.
• W4225577948 hasConceptScore W4225577948C178790620 @default.
• W4225577948 hasConceptScore W4225577948C185592680 @default.
• W4225577948 hasConceptScore W4225577948C202751555 @default.
• W4225577948 hasConceptScore W4225577948C203014093 @default.
• W4225577948 hasConceptScore W4225577948C207001950 @default.
• W4225577948 hasConceptScore W4225577948C26291073 @default.
• W4225577948 hasConceptScore W4225577948C2777714996 @default.
• W4225577948 hasConceptScore W4225577948C2777961210 @default.
• W4225577948 hasConceptScore W4225577948C2778754761 @default.

• next