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• W4225642711 abstract "Studying protein complexes in vitro requires the production of a relatively pure sample that maintains the full complement, native organization, and function of that complex. This can be particularly challenging to achieve for large, multi-component, membrane embedded complexes using the traditional recombinant expression and reconstitution methodologies. However, using affinity capture from native cells, suitable whole endogenous protein complexes can be isolated. Here we present a protocol for the affinity isolation of baker’s yeast (S. cerevisiae) nuclear pore complexes, which are ~50 MDa assemblies made up of 552 distinct proteins and embedded in a double-membraned nuclear envelope. Producing this sample allowed us for the first time to perform analyses to characterize the mass, stoichiometry, morphology, and connectivity of this complex and to obtain its integrative structure with ~9 Å precision. We believe this methodology can be applied to other challenging protein complexes to produce similar results." @default.
• W4225642711 created "2022-05-05" @default.
• W4225642711 creator A5036451354 @default.
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• W4225642711 date "2022-01-01" @default.
• W4225642711 modified "2023-10-07" @default.
• W4225642711 title "Affinity Isolation of Endogenous Saccharomyces Cerevisiae Nuclear Pore Complexes" @default.
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• W4225642711 doi "https://doi.org/10.1007/978-1-0716-2337-4_1" @default.
• W4225642711 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35412228" @default.
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