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- W4225814461 abstract "Mouse embryonic stem cells (mESCs) can be manipulated in vitro to recapitulate the process of erythropoiesis, during which multipotent cells undergo lineage specification, differentiation and maturation to produce erythroid cells. Although useful for identifying specific progenitors and precursors, this system has not been fully exploited as a source of cells to analyse erythropoiesis. Here, we establish a protocol in which characterised erythroblasts can be isolated in a scalable manner from differentiated embryoid bodies (EBs). Using transcriptional and epigenetic analysis, we demonstrate that this system faithfully recapitulates normal primitive erythropoiesis and fully reproduces the effects of natural and engineered mutations seen in primary cells obtained from mouse models. We anticipate this system to be of great value in reducing the time and costs of generating and maintaining mouse lines in a number of research scenarios." @default.
- W4225814461 created "2022-05-05" @default.
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- W4225814461 date "2022-01-07" @default.
- W4225814461 modified "2023-10-02" @default.
- W4225814461 title "Scalable in vitro production of defined mouse erythroblasts" @default.
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- W4225814461 doi "https://doi.org/10.1371/journal.pone.0261950" @default.
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- W4225814461 hasPublicationYear "2022" @default.
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