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• W4225819164 abstract "Abstract Small‐molecule binding assays to target proteins are a core component of drug discovery and development. While a number of assay formats are available, significant drawbacks still remain in cost, sensitivity, and throughput. To improve assays by capitalizing on the power of DNA sequence analysis, we have developed an assay method that combines DNA encoding with split‐and‐pool sample handling. The approach involves affinity labeling of DNA‐linked ligands to a protein target. Critically, the labeling event assesses ligand binding and enables subsequent pooling of several samples. Application of a purifying selection on the pool for protein‐labeled DNAs allows detection of ligand binding by quantification of DNA barcodes. We demonstrate the approach in both ligand displacement and direct binding formats and demonstrate its utility in determination of relative ligand affinity, profiling ligand specificity, and high‐throughput small‐molecule screening." @default.
• W4225819164 created "2022-05-05" @default.
• W4225819164 creator A5055516061 @default.
• W4225819164 creator A5065929661 @default.
• W4225819164 date "2021-12-03" @default.
• W4225819164 modified "2023-09-30" @default.
• W4225819164 title "Multiplexed Small Molecule Ligand Binding Assays by Affinity Labeling and DNA Sequence Analysis" @default.
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• W4225819164 doi "https://doi.org/10.1002/ange.202113515" @default.
• W4225819164 hasPublicationYear "2021" @default.
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