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• W4225999907 abstract "Rett syndrome (RTT) is a neurodevelopmental disorder that represents the most common genetic cause of severe intellectual disability in females. Most patients carry mutations in the X-linked MECP2 gene, coding for the methyl-CpG-binding protein 2 (MeCP2), originally isolated as an epigenetic transcriptional factor able to bind methylated DNA and repress transcription. Recent data implicated a role for glia in RTT, showing that astrocytes express Mecp2 and that its deficiency affects their ability to support neuronal maturation by non-cell autonomous mechanisms. To date, some molecular, structural and functional alterations have been attributed to Mecp2 null astrocytes, but how they evolve over time and whether they follow a spatial heterogeneity are two aspects which deserve further investigations. In this study, we assessed cytoskeletal features of astrocytes in Mecp2 deficient brains by analyzing their arbor complexity and processes in reconstructed GFAP+ cells at different ages, corresponding to peculiar stages of the disorder, and in different cerebral regions (motor and somatosensory cortices and CA1 layer of hippocampus). Our findings demonstrate the presence of defects in Mecp2 null astrocytes that worsen along disease progression and strictly depend on the brain area, highlighting motor and somatosensory cortices as the most affected regions. Of relevance, astrocyte cytoskeleton is impaired also in the somatosensory cortex of symptomatic heterozygous animals, with Mecp2+ astrocytes showing slightly more pronounced defects with respect to the Mecp2 null cells, emphasizing the importance of non-cell autonomous effects. We reported a temporal correlation between the progressive thinning of layer I and the atrophy of astrocytes, suggesting that their cytoskeletal dysfunctions might contribute to cortical defects. Considering the reciprocal link between morphology and function in astrocytes, we analyzed the effect of Mecp2 deficiency on the expression of selected astrocyte-enriched genes, which describe typical astrocytic features. qRT-PCR data corroborated our results, reporting an overall decrement of gene expression, which is area and age-dependent. In conclusion, our data show that Mecp2 deficiency causes structural and molecular alterations in astrocytes, which progress along with the severity of symptoms and diversely occur in the different cerebral regions, highlighting the importance of considering heterogeneity when studying astrocytes in RTT." @default.
• W4225999907 created "2022-05-05" @default.
• W4225999907 creator A5027274383 @default.
• W4225999907 creator A5029127362 @default.
• W4225999907 creator A5037162443 @default.
• W4225999907 creator A5055390568 @default.
• W4225999907 creator A5067024262 @default.
• W4225999907 creator A5079497661 @default.
• W4225999907 date "2022-02-15" @default.
• W4225999907 modified "2023-09-27" @default.
• W4225999907 title "Identification of Region-Specific Cytoskeletal and Molecular Alterations in Astrocytes of Mecp2 Deficient Animals" @default.
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• W4225999907 cites W1559742681 @default.
• W4225999907 cites W1566289870 @default.
• W4225999907 cites W1784857457 @default.
• W4225999907 cites W1786188576 @default.
• W4225999907 cites W1971735392 @default.
• W4225999907 cites W1977302167 @default.
• W4225999907 cites W1978127185 @default.
• W4225999907 cites W1987829135 @default.
• W4225999907 cites W1992789817 @default.
• W4225999907 cites W1994210488 @default.
• W4225999907 cites W1994974752 @default.
• W4225999907 cites W1995470989 @default.
• W4225999907 cites W2000313340 @default.
• W4225999907 cites W2001630973 @default.
• W4225999907 cites W2001936578 @default.
• W4225999907 cites W2013655994 @default.
• W4225999907 cites W2019864436 @default.
• W4225999907 cites W2026751327 @default.
• W4225999907 cites W2026778867 @default.
• W4225999907 cites W2039276157 @default.
• W4225999907 cites W2041174212 @default.
• W4225999907 cites W2045042214 @default.
• W4225999907 cites W2047585900 @default.
• W4225999907 cites W2048273592 @default.
• W4225999907 cites W2054275187 @default.
• W4225999907 cites W2074661552 @default.
• W4225999907 cites W2077563481 @default.
• W4225999907 cites W2098190939 @default.
• W4225999907 cites W2098684230 @default.
• W4225999907 cites W2101369162 @default.
• W4225999907 cites W2104054545 @default.
• W4225999907 cites W2105926864 @default.
• W4225999907 cites W2108470366 @default.
• W4225999907 cites W2123292451 @default.
• W4225999907 cites W2124940469 @default.
• W4225999907 cites W2127311839 @default.
• W4225999907 cites W2127621191 @default.
• W4225999907 cites W2129032286 @default.
• W4225999907 cites W2129977027 @default.
• W4225999907 cites W2134243901 @default.
• W4225999907 cites W2141410846 @default.
• W4225999907 cites W2143497500 @default.
• W4225999907 cites W2146997290 @default.
• W4225999907 cites W2151149439 @default.
• W4225999907 cites W2154057943 @default.
• W4225999907 cites W2154087863 @default.
• W4225999907 cites W2164502083 @default.
• W4225999907 cites W2168397438 @default.
• W4225999907 cites W2179188078 @default.
• W4225999907 cites W2179561038 @default.
• W4225999907 cites W2320983896 @default.
• W4225999907 cites W2341349973 @default.
• W4225999907 cites W2422132407 @default.
• W4225999907 cites W2526992340 @default.
• W4225999907 cites W2536209437 @default.
• W4225999907 cites W2559702793 @default.
• W4225999907 cites W2765768583 @default.
• W4225999907 cites W2777525962 @default.
• W4225999907 cites W2783672092 @default.
• W4225999907 cites W2795153441 @default.
• W4225999907 cites W2799543599 @default.
• W4225999907 cites W2801770351 @default.
• W4225999907 cites W2888094777 @default.
• W4225999907 cites W2941304317 @default.
• W4225999907 cites W2960111824 @default.
• W4225999907 cites W2969472499 @default.
• W4225999907 cites W2972571349 @default.
• W4225999907 cites W2980503825 @default.
• W4225999907 cites W3011286936 @default.
• W4225999907 cites W3015177924 @default.
• W4225999907 cites W3021594944 @default.
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• W4225999907 cites W3164684473 @default.
• W4225999907 cites W3200130362 @default.
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• W4225999907 doi "https://doi.org/10.3389/fnins.2022.823060" @default.
• W4225999907 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35242007" @default.
• W4225999907 hasPublicationYear "2022" @default.
• W4225999907 type Work @default.
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• W4225999907 hasAuthorship W4225999907A5027274383 @default.
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