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- W4226159082 abstract "In the past 15 years, optogenetic methods became invaluable tools in neurobiological research but also in general cell biology. Most prominently, optogenetic methods utilize microbial rhodopsins to elicit neuronal de- or hyperpolarization. However, other optogenetic tools have emerged that allow influencing neuronal function by different approaches. In this chapter we describe the use of photoactivated adenylyl cyclases (PACs) as modulators of neuronal activity. Using Caenorhabditis elegans as a model organism, this chapter shows how to measure the effect of PAC photoactivation by behavioral assays in different tissues (neurons and muscles), as well as their significance to neurobiology. Further, this chapter describes in vitro cyclic nucleoside-3',5'-monophosphate measurements (cNMP) to characterize new PACs in C. elegans." @default.
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- W4226159082 date "2022-01-01" @default.
- W4226159082 modified "2023-09-24" @default.
- W4226159082 title "Photoactivated Adenylyl Cyclases as Optogenetic Modulators of Neuronal Activity" @default.
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- W4226159082 doi "https://doi.org/10.1007/978-1-0716-2245-2_4" @default.
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