FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4226161330> ?p ?o ?g. }

• W4226161330 abstract "Vegetative insecticidal proteins (Vip3) from Bacillus thuringiensis have been used, in combination with Cry proteins, to better control insect pests and as a strategy to delay the evolution of resistance to Cry proteins in Bt crops (crops protected from insect attack by the expression of proteins from B. thuringiensis). In this study, we have set up the conditions to analyze the specific binding of 125I-Vip3Af to Spodoptera frugiperda and Spodoptera exigua brush border membrane vesicles (BBMV). Heterologous competition binding experiments revealed that Vip3Aa shares the same binding sites with Vip3Af, but Vip3Ca does not recognize all of them. As expected, Cry1Ac and Cry1F did not compete for Vip3Af binding sites. By trypsin treatment of selected alanine mutants, we were able to generate truncated versions of Vip3Af. Their use as competitors with 125I-Vip3Af indicated that only those molecules containing domains I to III (DI-III and DI-IV) were able to compete with the trypsin-activated Vip3Af protein for binding and that molecules only containing either domain IV or domains IV and V (DIV and DIV-V) were unable to compete with Vip3Af. These results were further confirmed with competition binding experiments using 125I-DI-III. In addition, the truncated protein 125I-DI-III also bound specifically to Sf21 cells. Cell viability assays showed that the truncated proteins DI-III and DI-IV were as toxic to Sf21 cells as the activated Vip3Af, suggesting that domains IV and V are not necessary for the toxicity to Sf21 cells, in contrast to their requirement in vivo.IMPORTANCE This study shows that Vip3Af binding sites are fully shared with Vip3Aa, only partially shared with Vip3Ca, and not shared with Cry1Ac and Cry1F in two Spodoptera spp. Truncated versions of Vip3Af revealed that only domains I to III were necessary for the specific binding, most likely because they can form the functional tetrameric oligomer and because domain III is supposed to contain the binding epitopes. In contrast to results obtained in vivo (bioassays against larvae), domains IV and V are not necessary for ex vivo toxicity to Sf21 cells." @default.
• W4226161330 created "2022-05-05" @default.
• W4226161330 creator A5011707883 @default.
• W4226161330 creator A5045539409 @default.
• W4226161330 creator A5070265750 @default.
• W4226161330 creator A5083844180 @default.
• W4226161330 date "2021-11-24" @default.
• W4226161330 modified "2023-10-09" @default.
• W4226161330 title "Critical Domains in the Specific Binding of Radiolabeled Vip3Af Insecticidal Protein to Brush Border Membrane Vesicles from <i>Spodoptera</i> spp. and Cultured Insect Cells" @default.
• W4226161330 cites W1508363397 @default.
• W4226161330 cites W1964844007 @default.
• W4226161330 cites W1988108636 @default.
• W4226161330 cites W1995853598 @default.
• W4226161330 cites W1997171318 @default.
• W4226161330 cites W2009418832 @default.
• W4226161330 cites W2012677194 @default.
• W4226161330 cites W2015491539 @default.
• W4226161330 cites W2023035494 @default.
• W4226161330 cites W2024018480 @default.
• W4226161330 cites W2027207844 @default.
• W4226161330 cites W2032777708 @default.
• W4226161330 cites W2035759078 @default.
• W4226161330 cites W2038960430 @default.
• W4226161330 cites W2061581594 @default.
• W4226161330 cites W2081335753 @default.
• W4226161330 cites W2086458348 @default.
• W4226161330 cites W2098634155 @default.
• W4226161330 cites W2102845745 @default.
• W4226161330 cites W2122707110 @default.
• W4226161330 cites W2128635872 @default.
• W4226161330 cites W2133621682 @default.
• W4226161330 cites W2134178361 @default.
• W4226161330 cites W2135217928 @default.
• W4226161330 cites W2142434901 @default.
• W4226161330 cites W2148238480 @default.
• W4226161330 cites W2153838526 @default.
• W4226161330 cites W2165442414 @default.
• W4226161330 cites W2174053989 @default.
• W4226161330 cites W2238168433 @default.
• W4226161330 cites W2290350278 @default.
• W4226161330 cites W2340151869 @default.
• W4226161330 cites W2488718415 @default.
• W4226161330 cites W2533247512 @default.
• W4226161330 cites W2588416884 @default.
• W4226161330 cites W2590951474 @default.
• W4226161330 cites W2607501182 @default.
• W4226161330 cites W2767537634 @default.
• W4226161330 cites W2770356453 @default.
• W4226161330 cites W2775448094 @default.
• W4226161330 cites W2803314076 @default.
• W4226161330 cites W2805579775 @default.
• W4226161330 cites W2807957048 @default.
• W4226161330 cites W2885809055 @default.
• W4226161330 cites W2887699478 @default.
• W4226161330 cites W2895264244 @default.
• W4226161330 cites W2897121426 @default.
• W4226161330 cites W2898483584 @default.
• W4226161330 cites W2899453626 @default.
• W4226161330 cites W2904850840 @default.
• W4226161330 cites W2945343217 @default.
• W4226161330 cites W2994903672 @default.
• W4226161330 cites W2996406654 @default.
• W4226161330 cites W3005401333 @default.
• W4226161330 cites W3008344251 @default.
• W4226161330 cites W3008964355 @default.
• W4226161330 cites W3036510235 @default.
• W4226161330 cites W3037739581 @default.
• W4226161330 cites W3040636142 @default.
• W4226161330 cites W3047665533 @default.
• W4226161330 cites W3049080316 @default.
• W4226161330 cites W3118338975 @default.
• W4226161330 cites W3162620234 @default.
• W4226161330 cites W3165147955 @default.
• W4226161330 cites W4229489656 @default.
• W4226161330 cites W4249042542 @default.
• W4226161330 cites W4293247451 @default.
• W4226161330 doi "https://doi.org/10.1128/aem.01787-21" @default.
• W4226161330 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/34586902" @default.
• W4226161330 hasPublicationYear "2021" @default.
• W4226161330 type Work @default.
• W4226161330 citedByCount "5" @default.
• W4226161330 countsByYear W42261613302022 @default.
• W4226161330 countsByYear W42261613302023 @default.
• W4226161330 crossrefType "journal-article" @default.
• W4226161330 hasAuthorship W4226161330A5011707883 @default.
• W4226161330 hasAuthorship W4226161330A5045539409 @default.
• W4226161330 hasAuthorship W4226161330A5070265750 @default.
• W4226161330 hasAuthorship W4226161330A5083844180 @default.
• W4226161330 hasBestOaLocation W42261613302 @default.
• W4226161330 hasConcept C102230213 @default.
• W4226161330 hasConcept C103878061 @default.
• W4226161330 hasConcept C104317684 @default.
• W4226161330 hasConcept C107824862 @default.
• W4226161330 hasConcept C122172344 @default.
• W4226161330 hasConcept C130316041 @default.
• W4226161330 hasConcept C153911025 @default.
• W4226161330 hasConcept C181199279 @default.
• W4226161330 hasConcept C2777773057 @default.
• W4226161330 hasConcept C2778457844 @default.
• W4226161330 hasConcept C2779035793 @default.

• next