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- W4226182576 endingPage "110346" @default.
- W4226182576 startingPage "110346" @default.
- W4226182576 abstract "Protein machines undergo conformational motions to interact with and manipulate polymeric substrates. The Sec translocase promiscuously recognizes, becomes activated, and secretes >500 non-folded preprotein clients across bacterial cytoplasmic membranes. Here, we reveal that the intrinsic dynamics of the translocase ATPase, SecA, and of preproteins combine to achieve translocation. SecA possesses an intrinsically dynamic preprotein clamp attached to an equally dynamic ATPase motor. Alternating motor conformations are finely controlled by the γ-phosphate of ATP, while ADP causes motor stalling, independently of clamp motions. Functional preproteins physically bridge these independent dynamics. Their signal peptides promote clamp closing; their mature domain overcomes the rate-limiting ADP release. While repeated ATP cycles shift the motor between unique states, multiple conformationally frustrated prongs in the clamp repeatedly catch and release trapped preprotein segments until translocation completion. This universal mechanism allows any preprotein to promiscuously recognize the translocase, usurp its intrinsic dynamics, and become secreted." @default.
- W4226182576 created "2022-05-05" @default.
- W4226182576 creator A5007167983 @default.
- W4226182576 creator A5011658666 @default.
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- W4226182576 creator A5030156142 @default.
- W4226182576 creator A5043039841 @default.
- W4226182576 creator A5046466799 @default.
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- W4226182576 creator A5058577851 @default.
- W4226182576 creator A5078253777 @default.
- W4226182576 creator A5087810702 @default.
- W4226182576 date "2022-02-01" @default.
- W4226182576 modified "2023-09-30" @default.
- W4226182576 title "Preproteins couple the intrinsic dynamics of SecA to its ATPase cycle to translocate via a catch and release mechanism" @default.
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- W4226182576 doi "https://doi.org/10.1016/j.celrep.2022.110346" @default.
- W4226182576 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35139375" @default.
- W4226182576 hasPublicationYear "2022" @default.