Matches in SemOpenAlex for { <https://semopenalex.org/work/W4226185458> ?p ?o ?g. }
- W4226185458 endingPage "225" @default.
- W4226185458 startingPage "191" @default.
- W4226185458 abstract "R-loop proteins present a stable and robust blockade to the progression of a DNA replication fork during S-phase. The consequences of this block can include mutagenesis and other irreversible chromosomal catastrophes, causing genomic instability and disease. As such, further investigation into the molecular mechanisms underlying R-loop protein resolution is warranted. The critical role of non-replicative accessory helicases in R-loop protein resolution has increasingly come into light in recent years. Such helicases include the Pif1-family, monomeric helicases that have been studied in many different contexts and that have been ascribed to a multitude of separable protective functions in the cell. In this chapter, we present protocols to study R-loop protein resolution by Pif1 helicase at stalled replication forks using purified proteins, both at the biochemical and single-molecule level. Our system uses recombinant proteins expressed in Saccharomyces cerevisiae but could apply to practically any organism of interest due to the high interspecies homology of the proteins involved in DNA replication. The methods we outline are extensible to many systems and should be applicable to studying R-loop clearance by any Superfamily (SF) 1B helicase. These techniques will further enable mechanistic research on these critical but understudied components of the genomic maintenance program." @default.
- W4226185458 created "2022-05-05" @default.
- W4226185458 creator A5022229776 @default.
- W4226185458 creator A5022463488 @default.
- W4226185458 creator A5057022114 @default.
- W4226185458 creator A5071520955 @default.
- W4226185458 date "2022-01-01" @default.
- W4226185458 modified "2023-09-28" @default.
- W4226185458 title "Biochemical and single-molecule techniques to study accessory helicase resolution of R-loop proteins at stalled replication forks" @default.
- W4226185458 cites W1482162053 @default.
- W4226185458 cites W1605838085 @default.
- W4226185458 cites W1968493165 @default.
- W4226185458 cites W1970732313 @default.
- W4226185458 cites W1970872037 @default.
- W4226185458 cites W1976892990 @default.
- W4226185458 cites W1985491895 @default.
- W4226185458 cites W1989379056 @default.
- W4226185458 cites W1990897746 @default.
- W4226185458 cites W2003719212 @default.
- W4226185458 cites W2012598582 @default.
- W4226185458 cites W2030974598 @default.
- W4226185458 cites W2042969047 @default.
- W4226185458 cites W2043745733 @default.
- W4226185458 cites W2044440192 @default.
- W4226185458 cites W2048897962 @default.
- W4226185458 cites W2052257191 @default.
- W4226185458 cites W2053259460 @default.
- W4226185458 cites W2056463455 @default.
- W4226185458 cites W2058654089 @default.
- W4226185458 cites W2060262536 @default.
- W4226185458 cites W2079991169 @default.
- W4226185458 cites W2094569502 @default.
- W4226185458 cites W2100032426 @default.
- W4226185458 cites W2110932617 @default.
- W4226185458 cites W2120628590 @default.
- W4226185458 cites W2166150079 @default.
- W4226185458 cites W2167279371 @default.
- W4226185458 cites W2226186365 @default.
- W4226185458 cites W2275043758 @default.
- W4226185458 cites W2312944452 @default.
- W4226185458 cites W2398139621 @default.
- W4226185458 cites W2473838984 @default.
- W4226185458 cites W2514550219 @default.
- W4226185458 cites W2515159273 @default.
- W4226185458 cites W2531499154 @default.
- W4226185458 cites W2565773396 @default.
- W4226185458 cites W2566002255 @default.
- W4226185458 cites W2568610139 @default.
- W4226185458 cites W2591019284 @default.
- W4226185458 cites W2594597600 @default.
- W4226185458 cites W2606203505 @default.
- W4226185458 cites W2743954317 @default.
- W4226185458 cites W2750812953 @default.
- W4226185458 cites W2754218973 @default.
- W4226185458 cites W2754244753 @default.
- W4226185458 cites W2754763401 @default.
- W4226185458 cites W2769509030 @default.
- W4226185458 cites W2787975097 @default.
- W4226185458 cites W2788084906 @default.
- W4226185458 cites W2790768166 @default.
- W4226185458 cites W2883755935 @default.
- W4226185458 cites W2899721343 @default.
- W4226185458 cites W2900339116 @default.
- W4226185458 cites W2906523411 @default.
- W4226185458 cites W2913346540 @default.
- W4226185458 cites W2915069273 @default.
- W4226185458 cites W2919983752 @default.
- W4226185458 cites W2944977287 @default.
- W4226185458 cites W2945567049 @default.
- W4226185458 cites W2949919700 @default.
- W4226185458 cites W2952320792 @default.
- W4226185458 cites W2953339106 @default.
- W4226185458 cites W2959460977 @default.
- W4226185458 cites W2964264171 @default.
- W4226185458 cites W2973731348 @default.
- W4226185458 cites W2974182118 @default.
- W4226185458 cites W2979531443 @default.
- W4226185458 cites W2989260338 @default.
- W4226185458 cites W2989677681 @default.
- W4226185458 cites W2990967805 @default.
- W4226185458 cites W2995195036 @default.
- W4226185458 cites W3006859342 @default.
- W4226185458 cites W3025358337 @default.
- W4226185458 cites W3085850424 @default.
- W4226185458 cites W3098744506 @default.
- W4226185458 cites W3121004564 @default.
- W4226185458 cites W3124798421 @default.
- W4226185458 cites W3126350730 @default.
- W4226185458 cites W3154159901 @default.
- W4226185458 cites W3166745924 @default.
- W4226185458 cites W3173330266 @default.
- W4226185458 cites W3188853485 @default.
- W4226185458 cites W3193400220 @default.
- W4226185458 cites W3197346238 @default.
- W4226185458 cites W3212521059 @default.
- W4226185458 cites W4200214832 @default.
- W4226185458 cites W4240412933 @default.
- W4226185458 cites W66535291 @default.