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• W4226332012 abstract "The pathogenic bacteria have been found to reside within the macrophages wherein they are controlled by the later. However, the recent data has shown that human granulocytes have an important contribution in regulating innate immunity during tuberculosis as a result of the different granulocyte proteins they contain within them. In this study, we have isolated and identified a few from human granulocytes and checked its efficacy against M. smegmatis and M. bovis BCG respectively. Using the protocol for gel overlay assay and the colony forming units (CFU) assay, we showed that out of the three identified proteins, that is, hemoglobin, lactoferrin, and lipocalin, lactoferrin has been found to be a potential antimycobactericidal molecule. These proteins were identified through MALDI-TOF mass spectrometry analysis. Extracellular addition of these proteins in different doses to the mouse macrophage RAW 264.7 and human monocyte cell line THP-1 showed significant reduction in intracellular mycobacterial survival without any significant cytotoxicity on the cell lines. An ESAT-6 family member protein Rv2346c of Mycobacterium tuberculosis has been cloned and expressed in a nonpathogenic host M. smegmatis which has more survival within the mouse macrophages cell line (RAW 264.7) and human monocyte macrophage (THP-1) by inducing host cell death upon infection and also upregulated production of nitric oxide (NO), reactive oxygen species (ROS), catalase, and superoxide ions, respectively. Macrophages treated with lactoferrin significantly enhanced cell viability by reducing the percentage of apoptotic cells and reduced oxidative stress damage to host cells. From this data, it can be concluded that human granulocyte proteins may play a very integral role in mediating intracellular killing of mycobacteria within macrophages thereby inducing host cell viability." @default.
• W4226332012 created "2022-05-05" @default.
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• W4226332012 date "2022-01-01" @default.
• W4226332012 modified "2023-09-25" @default.
• W4226332012 title "Human granulocyte proteins constitute the major antimycobactericidal proteins and enhance the killing of mycobacteria within macrophages" @default.
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• W4226332012 doi "https://doi.org/10.1016/b978-0-323-91251-8.00009-x" @default.
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