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- W4226343352 abstract "During post-transcriptional gene regulation (PTGR), RNA binding proteins (RBPs) interact with all classes of RNA to control RNA maturation, stability, transport, and translation. Here, we describe Photoactivatable-Ribonucleoside-Enhanced Crosslinking and Immunoprecipitation (PAR-CLIP), a transcriptome-scale method for identifying RBP binding sites on target RNAs with nucleotide-level resolution. This method is readily applicable to any protein directly contacting RNA, including RBPs that are predicted to bind in a sequence- or structure-dependent manner at discrete RNA recognition elements (RREs), and those that are thought to bind transiently, such as RNA polymerases or helicases." @default.
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- W4226343352 date "2021-10-26" @default.
- W4226343352 modified "2023-09-27" @default.
- W4226343352 title "PAR-CLIP: A Method for Transcriptome-Wide Identification of RNA Binding Protein Interaction Sites" @default.
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- W4226343352 doi "https://doi.org/10.1007/978-1-0716-1851-6_9" @default.
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