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- W4226392775 abstract "The aim of the study was to analyze the proteome of the wheat (Triticum aestivum L.), oat (Avena sativa) and buckwheat (Fagopyrum esculentum Moench.) genotypes using immunochemical methods and to detect the presence of celiac active polypeptides. The results of ELISA confirmed a high gluten content in wheat (43,278.51 mg·kg−1). The buckwheat varieties (average 2.03 mg·kg−1) fulfill the criteria for labeling as gluten-free food. The results confirm the oat varietal dependence on the content of allergenic proteins. The Western blot analysis revealed the strongest reactions of the polyclonal antibody with wheat proteins of 25 kDa to 50 kDa and with oat proteins of 20 kDa and 40–55 kDa. No signal was detected in buckwheat, which was confirmed by ELISA results. Buckwheat can be recommended as a safe crop for the preparation of gluten-free foods." @default.
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- W4226392775 date "2021-11-30" @default.
- W4226392775 modified "2023-10-16" @default.
- W4226392775 title "Detection of Celiac Active Polypeptides in Wheat, Oat and Buckwheat Using Immunochemical Methods" @default.
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- W4226392775 doi "https://doi.org/10.3390/iecps2021-11976" @default.
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