Matches in SemOpenAlex for { <https://semopenalex.org/work/W4229012249> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W4229012249 endingPage "144" @default.
- W4229012249 startingPage "138" @default.
- W4229012249 abstract "This study aimed to screen anaplasia-related genes that influence the progression of retinoblastoma (RB) and to identify immune cells associated with the poor prognosis.Differentially expressed genes (DEGs) between retina and RB samples were acquired from gene expression omnibus (GEO) database. Candidate hub genes were screened by taking intersections among the co-expressed genes, the hub nodes, and DEGs of the validation set. The hub genes were identified by receiver operating characteristic (ROC) and quantitative real-time PCR (qPCR). Immune infiltration levels of RB tissues were estimated using single-sample gene set enrichment analysis (ssGSEA). The functions of RB cells were detected by CCK8, EDU and flow cytometry assays.665 DEGs involved in the genesis and progression of RB were acquired from GEO database. 29 candidate hub genes were screened by examining 43 co-expressed genes and 63 hub nodes. 9 hub genes (CHEK1, EXO1, FANCI, GTSE1, MELK, MKI67, NCAPH, PRC1, and UBE2T) strongly related to the anaplastic grades were validated by ROC curve analysis (AUC >0.8). Based on the ssGSEA scores, the immune infiltration levels of Th2 cells were positively associated with anaplastic grade. qPCR assay showed that 9 hub genes were upregulated in RB cells, and UBE2T expressed remarkably high. CCK 8, EDU, and flow cytometry assays revealed that UBE2T silencing inhibited the proliferation of RB cells and incited apoptosis.The increased infiltration of Th2 cells and upregulated expression of 9 hub genes predict a poor prognosis of RB. UBE2T can be a therapeutic target for RB treatment." @default.
- W4229012249 created "2022-05-08" @default.
- W4229012249 creator A5014579494 @default.
- W4229012249 creator A5014792449 @default.
- W4229012249 creator A5022256556 @default.
- W4229012249 creator A5037361773 @default.
- W4229012249 creator A5038401031 @default.
- W4229012249 creator A5040211589 @default.
- W4229012249 creator A5056407602 @default.
- W4229012249 date "2022-07-01" @default.
- W4229012249 modified "2023-10-16" @default.
- W4229012249 title "UBE2T is a prognostic biomarker and correlated with Th2 cell infiltrates in retinoblastoma" @default.
- W4229012249 cites W1966107523 @default.
- W4229012249 cites W2122122620 @default.
- W4229012249 cites W2142470584 @default.
- W4229012249 cites W2146512944 @default.
- W4229012249 cites W2159707944 @default.
- W4229012249 cites W2604951349 @default.
- W4229012249 cites W2761824327 @default.
- W4229012249 cites W2790774242 @default.
- W4229012249 cites W2883052277 @default.
- W4229012249 cites W2884967698 @default.
- W4229012249 cites W2949248283 @default.
- W4229012249 cites W2953442466 @default.
- W4229012249 cites W2955745836 @default.
- W4229012249 cites W2974883295 @default.
- W4229012249 cites W3006227213 @default.
- W4229012249 cites W3037862218 @default.
- W4229012249 cites W3092497389 @default.
- W4229012249 cites W3094054958 @default.
- W4229012249 cites W3095371442 @default.
- W4229012249 cites W3128235844 @default.
- W4229012249 cites W3186276457 @default.
- W4229012249 cites W3204022284 @default.
- W4229012249 cites W3216491528 @default.
- W4229012249 cites W4200071890 @default.
- W4229012249 doi "https://doi.org/10.1016/j.bbrc.2022.04.096" @default.
- W4229012249 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35594577" @default.
- W4229012249 hasPublicationYear "2022" @default.
- W4229012249 type Work @default.
- W4229012249 citedByCount "1" @default.
- W4229012249 countsByYear W42290122492023 @default.
- W4229012249 crossrefType "journal-article" @default.
- W4229012249 hasAuthorship W4229012249A5014579494 @default.
- W4229012249 hasAuthorship W4229012249A5014792449 @default.
- W4229012249 hasAuthorship W4229012249A5022256556 @default.
- W4229012249 hasAuthorship W4229012249A5037361773 @default.
- W4229012249 hasAuthorship W4229012249A5038401031 @default.
- W4229012249 hasAuthorship W4229012249A5040211589 @default.
- W4229012249 hasAuthorship W4229012249A5056407602 @default.
- W4229012249 hasConcept C104317684 @default.
- W4229012249 hasConcept C119056186 @default.
- W4229012249 hasConcept C127561419 @default.
- W4229012249 hasConcept C153911025 @default.
- W4229012249 hasConcept C203014093 @default.
- W4229012249 hasConcept C2776577112 @default.
- W4229012249 hasConcept C502942594 @default.
- W4229012249 hasConcept C54355233 @default.
- W4229012249 hasConcept C553184892 @default.
- W4229012249 hasConcept C86803240 @default.
- W4229012249 hasConcept C8891405 @default.
- W4229012249 hasConceptScore W4229012249C104317684 @default.
- W4229012249 hasConceptScore W4229012249C119056186 @default.
- W4229012249 hasConceptScore W4229012249C127561419 @default.
- W4229012249 hasConceptScore W4229012249C153911025 @default.
- W4229012249 hasConceptScore W4229012249C203014093 @default.
- W4229012249 hasConceptScore W4229012249C2776577112 @default.
- W4229012249 hasConceptScore W4229012249C502942594 @default.
- W4229012249 hasConceptScore W4229012249C54355233 @default.
- W4229012249 hasConceptScore W4229012249C553184892 @default.
- W4229012249 hasConceptScore W4229012249C86803240 @default.
- W4229012249 hasConceptScore W4229012249C8891405 @default.
- W4229012249 hasFunder F4320321001 @default.
- W4229012249 hasLocation W42290122491 @default.
- W4229012249 hasLocation W42290122492 @default.
- W4229012249 hasOpenAccess W4229012249 @default.
- W4229012249 hasPrimaryLocation W42290122491 @default.
- W4229012249 hasRelatedWork W1640246868 @default.
- W4229012249 hasRelatedWork W2001236824 @default.
- W4229012249 hasRelatedWork W2296384492 @default.
- W4229012249 hasRelatedWork W2540716486 @default.
- W4229012249 hasRelatedWork W2604918044 @default.
- W4229012249 hasRelatedWork W2971307441 @default.
- W4229012249 hasRelatedWork W3083989164 @default.
- W4229012249 hasRelatedWork W3203495796 @default.
- W4229012249 hasRelatedWork W4224232191 @default.
- W4229012249 hasRelatedWork W4322723986 @default.
- W4229012249 hasVolume "614" @default.
- W4229012249 isParatext "false" @default.
- W4229012249 isRetracted "false" @default.
- W4229012249 workType "article" @default.