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- W4229076305 endingPage "109967" @default.
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- W4229076305 abstract "Esophageal cancer is the seventh most common cancer globally. Chemotherapy resistance remains a significant challenge in the treatment of esophageal cancer patients. Cisplatin can damage tumor cells by inducing pyroptosis. However, the underlying molecular mechanisms remain unclear. In this work, we aim to investigate pyroptosis-dependent molecular mechanisms underlying cisplatin sensitivity and find potential biomarkers to predict response to cisplatin-based chemotherapy for esophageal cancer patients. Pyroptosis-associated proteins were screened via proteomics for esophageal cancer (n = 124) and bioinformatics analysis. We observed that high calpain-1 (CAPN1) and calpain-2 (CAPN2) expression were associated with favorable clinical outcomes and prolonged survival in esophageal cancer patients. We employed immunohistochemistry to evaluate the expression of CAPN1 and CAPN2 in pretreatment tumor biopsies from 108 patients with esophageal cancer who received concurrent chemoradiotherapy (CCRT). These results suggested that esophageal cancer patients with high expression of both CAPN1 and CAPN2 are likely to experience a complete response to CCRT and have significantly better survival. Western blotting, LDH release, calpain activity and cell viability assays indicated that cisplatin could activate calpain activity, while calpain inhibition or knockout suppressed cisplatin-induced pyroptosis. Mechanistically, we uncovered a novel mechanism whereby cisplatin induced pyroptosis via activation of a CAPN1/CAPN2-BAK/BAX-caspase-9-caspase-3-GSDME signaling axis in esophageal cancer cells. Collectively, this study is the first to explore the effects of calpain on cisplatin-induced pyroptosis in esophageal cancer cells. Further, our findings also imply that the combination of CAPN1 and CAPN2 could be considered as a promising biomarker of cisplatin sensitivity and prognosis in patients with esophageal cancer, providing a possibility to guide individualized treatment." @default.
- W4229076305 created "2022-05-08" @default.
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- W4229076305 date "2022-07-01" @default.
- W4229076305 modified "2023-10-16" @default.
- W4229076305 title "Cisplatin-induced pyroptosis is mediated via the CAPN1/CAPN2-BAK/BAX-caspase-9-caspase-3-GSDME axis in esophageal cancer" @default.
- W4229076305 cites W1521822265 @default.
- W4229076305 cites W1597027218 @default.
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- W4229076305 cites W1966081420 @default.
- W4229076305 cites W1995041486 @default.
- W4229076305 cites W1995889458 @default.
- W4229076305 cites W2001656004 @default.
- W4229076305 cites W2011859773 @default.
- W4229076305 cites W2019156893 @default.
- W4229076305 cites W2029797799 @default.
- W4229076305 cites W2038062002 @default.
- W4229076305 cites W2059246452 @default.
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- W4229076305 cites W2065382366 @default.
- W4229076305 cites W2073131323 @default.
- W4229076305 cites W2073914642 @default.
- W4229076305 cites W2080467439 @default.
- W4229076305 cites W2080982503 @default.
- W4229076305 cites W2088883749 @default.
- W4229076305 cites W2090137644 @default.
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- W4229076305 cites W2133650808 @default.
- W4229076305 cites W2134230722 @default.
- W4229076305 cites W2135502832 @default.
- W4229076305 cites W2162129881 @default.
- W4229076305 cites W2169436026 @default.
- W4229076305 cites W2252711380 @default.
- W4229076305 cites W2309904837 @default.
- W4229076305 cites W2341790966 @default.
- W4229076305 cites W2462773201 @default.
- W4229076305 cites W2465392781 @default.
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- W4229076305 cites W2755217462 @default.
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- W4229076305 cites W2788640168 @default.
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- W4229076305 cites W2885846830 @default.
- W4229076305 cites W2904577546 @default.
- W4229076305 cites W2911937431 @default.
- W4229076305 cites W2920262430 @default.
- W4229076305 cites W2922122232 @default.
- W4229076305 cites W2936835725 @default.
- W4229076305 cites W2953512464 @default.
- W4229076305 cites W2957409472 @default.
- W4229076305 cites W2959952467 @default.
- W4229076305 cites W2967680982 @default.
- W4229076305 cites W3006826877 @default.
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- W4229076305 cites W3128646645 @default.
- W4229076305 cites W3131879536 @default.
- W4229076305 cites W3136325692 @default.
- W4229076305 cites W3183286210 @default.
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- W4229076305 doi "https://doi.org/10.1016/j.cbi.2022.109967" @default.
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