FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4229333803> ?p ?o ?g. }

• W4229333803 endingPage "3209" @default.
• W4229333803 startingPage "3209" @default.
• W4229333803 abstract "Pennycress (Thlaspi arvense L.), an annual herb of the mustard family Brassicacae, is native to Eurasia and now widely distributed throughout temperate North America. This species is currently being developed as a medicinal herb used to treat nephritis in China and an oilseed crop for biofuel production (Roque et al.2012). In November 2020, stunt and wilt symptoms were observed on above ground parts and swollen club-shaped galls were observed on the roots of T. arvense in most of the Chinese cabbage growing area in Kangding (30°03'N,102°02'E), Sichuan Province of China. The average disease incidence of swollen roots on T. arvense was 91.2% ( n=80). To identify the causal agent of this disease, the swollen roots of T. arvense were collected, crushed and observed under microscope (Fei et al.2017). Abundant resting spores were found in the root galls, which were spherical and 2.0 to 3.1 μm in diameter with an average length of 2.7 nm (n=100). The healthy roots and the root galls of T. arvense plants were further evaluated by PCR with P. brassicae-specific primers TC2F/TC2R (Cao et al. 2007). The results showed that a DNA fragment with an estimated size of 520 bp, as expected for that of P. brassicae, was consistently amplified in diseased roots, No PCR amplification occurred in the healthy roots with the TC2F/TC2R primers. Blast analysis of the 520 bp segment (GenBankMZ040496) showed the highest identity with the sequence of small subunit ribosomal RNA gene of P. brassicae (GenBankMH762161, 97.7%, E value=0). These results confirmed that the pathogen in the galled roots of T. arvense was P. brassicae. The pathogenicity of isolated P. brassicae was tested on both T. arvense and Chinese cabbage (B. campestris ssp. pekinensis). Resting spores were isolated from the diseased roots (Castlkbury et al. 1994) of T. arvense and suspended in Hoagland's solution to the final concentration of 1 × 107 spores per milliliter. Fifteen plastic pots (10 cm bottom diameter, 16 cm upper diameter, 13 cm high) were filled with soil (1 kg per pot) that was sterilized twice with high-temperature (121℃), high pressure (19 PSI) for 1.5 hours with a time interval of 2 days between. Inoculated pots received 100 mL spore suspension each. Fifteen control pots with sterilized field soil were treated with 100 mL Hoagland's solution each. Seeds of T. arvense and B. campestris were pre-germinated at 20°C on moist filter paper for 7 days and transplanted into the pots, five seedlings each and five pots per treatment. The pots were maintained in a greenhouse with 16 hours photoperiod at 24°C/16°C day/night temperature. After 7 weeks, plants in each pot were uprooted and the roots cleaned in running water and inspected for clubroot symptoms. Plants of T. arvense and Chinese cabbage in pots inoculated with resting spores showed clubroot symptoms while no disease symptoms were observed on any control plants. The disease incidence rate was 95.4% on T. arvense and 81.2% on B. campestris. Therefore, it was confirmed that P. brassicae could cause clubroot disease on T. arvense. To our knowledge, this is the first published report of clubroot disease on T. arvense in China. This finding is helpful for the management of clubroot on herbs and plants of biological origin in the cruciferous family." @default.
• W4229333803 created "2022-05-09" @default.
• W4229333803 creator A5003903279 @default.
• W4229333803 creator A5029526258 @default.
• W4229333803 creator A5031642533 @default.
• W4229333803 creator A5033080900 @default.
• W4229333803 creator A5071798264 @default.
• W4229333803 creator A5083294195 @default.
• W4229333803 creator A5016140592 @default.
• W4229333803 date "2022-12-01" @default.
• W4229333803 modified "2023-10-02" @default.
• W4229333803 title "First Report of <i>Plasmodiophora brassicae</i> Causing Clubroot on <i>Thlaspi arvense</i> in Sichuan Province of China" @default.
• W4229333803 cites W1974114122 @default.
• W4229333803 cites W2086602947 @default.
• W4229333803 cites W2171422009 @default.
• W4229333803 doi "https://doi.org/10.1094/pdis-12-21-2763-pdn" @default.
• W4229333803 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35522963" @default.
• W4229333803 hasPublicationYear "2022" @default.
• W4229333803 type Work @default.
• W4229333803 citedByCount "1" @default.
• W4229333803 countsByYear W42293338032023 @default.
• W4229333803 crossrefType "journal-article" @default.
• W4229333803 hasAuthorship W4229333803A5003903279 @default.
• W4229333803 hasAuthorship W4229333803A5016140592 @default.
• W4229333803 hasAuthorship W4229333803A5029526258 @default.
• W4229333803 hasAuthorship W4229333803A5031642533 @default.
• W4229333803 hasAuthorship W4229333803A5033080900 @default.
• W4229333803 hasAuthorship W4229333803A5071798264 @default.
• W4229333803 hasAuthorship W4229333803A5083294195 @default.
• W4229333803 hasConcept C137580998 @default.
• W4229333803 hasConcept C174618031 @default.
• W4229333803 hasConcept C2775891814 @default.
• W4229333803 hasConcept C2776703688 @default.
• W4229333803 hasConcept C2776747608 @default.
• W4229333803 hasConcept C2777451525 @default.
• W4229333803 hasConcept C2779016451 @default.
• W4229333803 hasConcept C2779824472 @default.
• W4229333803 hasConcept C2991655182 @default.
• W4229333803 hasConcept C556039675 @default.
• W4229333803 hasConcept C59822182 @default.
• W4229333803 hasConcept C6557445 @default.
• W4229333803 hasConcept C71924100 @default.
• W4229333803 hasConcept C86803240 @default.
• W4229333803 hasConceptScore W4229333803C137580998 @default.
• W4229333803 hasConceptScore W4229333803C174618031 @default.
• W4229333803 hasConceptScore W4229333803C2775891814 @default.
• W4229333803 hasConceptScore W4229333803C2776703688 @default.
• W4229333803 hasConceptScore W4229333803C2776747608 @default.
• W4229333803 hasConceptScore W4229333803C2777451525 @default.
• W4229333803 hasConceptScore W4229333803C2779016451 @default.
• W4229333803 hasConceptScore W4229333803C2779824472 @default.
• W4229333803 hasConceptScore W4229333803C2991655182 @default.
• W4229333803 hasConceptScore W4229333803C556039675 @default.
• W4229333803 hasConceptScore W4229333803C59822182 @default.
• W4229333803 hasConceptScore W4229333803C6557445 @default.
• W4229333803 hasConceptScore W4229333803C71924100 @default.
• W4229333803 hasConceptScore W4229333803C86803240 @default.
• W4229333803 hasIssue "12" @default.
• W4229333803 hasLocation W42293338031 @default.
• W4229333803 hasLocation W42293338032 @default.
• W4229333803 hasOpenAccess W4229333803 @default.
• W4229333803 hasPrimaryLocation W42293338031 @default.
• W4229333803 hasRelatedWork W1573837706 @default.
• W4229333803 hasRelatedWork W1789638574 @default.
• W4229333803 hasRelatedWork W2009379506 @default.
• W4229333803 hasRelatedWork W2063398372 @default.
• W4229333803 hasRelatedWork W2086961301 @default.
• W4229333803 hasRelatedWork W2157796563 @default.
• W4229333803 hasRelatedWork W2170169411 @default.
• W4229333803 hasRelatedWork W2888798802 @default.
• W4229333803 hasRelatedWork W2914795828 @default.
• W4229333803 hasRelatedWork W3161689889 @default.
• W4229333803 hasVolume "106" @default.
• W4229333803 isParatext "false" @default.
• W4229333803 isRetracted "false" @default.
• W4229333803 workType "article" @default.