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• W4229334422 abstract "Abstract Background In the yeast Saccharomyces cerevisiae , which is widely applied for industrial bioethanol production, uptake of hexoses is mediated by transporters with a facilitated diffusion mechanism. In anaerobic cultures, a higher ethanol yield can be achieved when transport of hexoses is proton-coupled, because of the lower net ATP yield of sugar dissimilation. In this study, the facilitated diffusion transport system for hexose sugars of S. cerevisiae was replaced by hexose–proton symport. Results Introduction of heterologous glucose– or fructose–proton symporters in an hxt 0 yeast background strain (derived from CEN.PK2-1C) restored growth on the corresponding sugar under aerobic conditions. After applying an evolutionary engineering strategy to enable anaerobic growth, the hexose–proton symporter-expressing strains were grown in anaerobic, hexose-limited chemostats on synthetic defined medium, which showed that the biomass yield of the resulting strains was decreased by 44.0-47.6%, whereas the ethanol yield had increased by up to 17.2% (from 1.51 to 1.77 mol mol hexose −1 ) compared to an isogenic strain expressing the hexose uniporter HXT5 . To apply this strategy to increase the ethanol yield on sucrose, we constructed a platform strain in which all genes encoding hexose transporters, disaccharide transporters and disaccharide hydrolases were deleted, after which a combination of a glucose–proton symporter, fructose–proton symporter and extracellular invertase ( SUC2 ) were introduced. After evolution, the resulting strain exhibited a 16.6% increased anaerobic ethanol yield (from 1.51 to 1.76 mol mol hexose equivalent −1 ) and 46.6% decreased biomass yield on sucrose. Conclusions This study provides a proof-of-concept for the replacement of the endogenous hexose transporters of S. cerevisiae by hexose-proton symport, and the concomitant decrease in ATP yield, to greatly improve the anaerobic yield of ethanol on sugar. Moreover, the sugar-negative platform strain constructed in this study acts as a valuable starting point for future studies on sugar transport or development of cell factories requiring specific sugar transport mechanisms." @default.
• W4229334422 created "2022-05-09" @default.
• W4229334422 creator A5008826623 @default.
• W4229334422 creator A5030683254 @default.
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• W4229334422 creator A5069488232 @default.
• W4229334422 date "2022-05-07" @default.
• W4229334422 modified "2023-10-18" @default.
• W4229334422 title "Engineering proton-coupled hexose uptake in Saccharomyces cerevisiae for improved ethanol yield" @default.
• W4229334422 cites W1483456869 @default.
• W4229334422 cites W1507531440 @default.
• W4229334422 cites W1547518044 @default.
• W4229334422 cites W1555560879 @default.
• W4229334422 cites W1754334190 @default.
• W4229334422 cites W1804531429 @default.
• W4229334422 cites W1850569493 @default.
• W4229334422 cites W1965505095 @default.
• W4229334422 cites W1967536663 @default.
• W4229334422 cites W1968463000 @default.
• W4229334422 cites W1977404998 @default.
• W4229334422 cites W1979386761 @default.
• W4229334422 cites W1981919911 @default.
• W4229334422 cites W1992824986 @default.
• W4229334422 cites W1997664932 @default.
• W4229334422 cites W2004595816 @default.
• W4229334422 cites W2008141632 @default.
• W4229334422 cites W2008270905 @default.
• W4229334422 cites W2016033442 @default.
• W4229334422 cites W201639086 @default.
• W4229334422 cites W2035215420 @default.
• W4229334422 cites W2035314369 @default.
• W4229334422 cites W2039609168 @default.
• W4229334422 cites W2041946854 @default.
• W4229334422 cites W2047049388 @default.
• W4229334422 cites W2052078471 @default.
• W4229334422 cites W2054061030 @default.
• W4229334422 cites W2054496945 @default.
• W4229334422 cites W2061510626 @default.
• W4229334422 cites W2067927484 @default.
• W4229334422 cites W2069857552 @default.
• W4229334422 cites W2084338561 @default.
• W4229334422 cites W2086922902 @default.
• W4229334422 cites W2089891952 @default.
• W4229334422 cites W2093320542 @default.
• W4229334422 cites W2097588241 @default.
• W4229334422 cites W2097666362 @default.
• W4229334422 cites W2098613540 @default.
• W4229334422 cites W2099257216 @default.
• W4229334422 cites W2101442503 @default.
• W4229334422 cites W2104898615 @default.
• W4229334422 cites W2115420193 @default.
• W4229334422 cites W2115425788 @default.
• W4229334422 cites W2118625346 @default.
• W4229334422 cites W2122113339 @default.
• W4229334422 cites W2123782813 @default.
• W4229334422 cites W2128138589 @default.
• W4229334422 cites W2138350421 @default.
• W4229334422 cites W2138664060 @default.
• W4229334422 cites W2145944929 @default.
• W4229334422 cites W2146843013 @default.
• W4229334422 cites W2149525061 @default.
• W4229334422 cites W2151258701 @default.
• W4229334422 cites W2153223092 @default.
• W4229334422 cites W2153770183 @default.
• W4229334422 cites W2154018668 @default.
• W4229334422 cites W2162056846 @default.
• W4229334422 cites W2165418074 @default.
• W4229334422 cites W2169333620 @default.
• W4229334422 cites W2171652642 @default.
• W4229334422 cites W2297853161 @default.
• W4229334422 cites W2464079324 @default.
• W4229334422 cites W2507537832 @default.
• W4229334422 cites W2511566537 @default.
• W4229334422 cites W2624753255 @default.
• W4229334422 cites W2682482079 @default.
• W4229334422 cites W2768684613 @default.
• W4229334422 cites W2771530540 @default.
• W4229334422 cites W2796932002 @default.
• W4229334422 cites W2895435534 @default.
• W4229334422 cites W2902733144 @default.
• W4229334422 cites W2909239313 @default.
• W4229334422 cites W2969306840 @default.
• W4229334422 cites W2982453414 @default.
• W4229334422 cites W2989941198 @default.
• W4229334422 cites W3046397622 @default.
• W4229334422 cites W3128089274 @default.
• W4229334422 cites W3173568189 @default.
• W4229334422 cites W4206931228 @default.
• W4229334422 cites W4255280485 @default.
• W4229334422 cites W70082989 @default.
• W4229334422 doi "https://doi.org/10.1186/s13068-022-02145-7" @default.
• W4229334422 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35524322" @default.
• W4229334422 hasPublicationYear "2022" @default.
• W4229334422 type Work @default.
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• W4229334422 hasAuthorship W4229334422A5030683254 @default.

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