FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4229371010> ?p ?o ?g. }

• W4229371010 abstract "Abstract Certain species within the genus Pseudo-nitzschia are able to produce the neurotoxin domoic acid (DA), which can cause illness in humans, mass-mortality of marine animals, and closure of commercial and recreational shellfisheries during toxic events. Understanding and forecasting blooms of these harmful species is a primary management goal. However, accurately predicting the onset and severity of bloom events remains difficult, in part because the underlying drivers of bloom formation have not been fully resolved. Furthermore, Pseudo-nitzschia species often co-occur, and recent work suggests that the genetic composition of a Pseudo-nitzschia bloom may be a better predictor of toxicity than prevailing environmental conditions. We developed a novel next-generation sequencing assay using restriction site-associated DNA (2b-RAD) genotyping and applied it to mock Pseudo-nitzschia communities generated by mixing cultures of different species in known abundances. On average, 94% of the variance in observed species abundance was explained by the expected abundance. In addition, the false positive rate was low (0.45% on average) and unrelated to read depth, and false negatives were never observed. Application of this method to environmental DNA samples collected during natural Pseudo-nitzschia spp. bloom events in Southern California revealed that increases in DA were associated with increases in the relative abundance of P. australis . Although the absolute correlation across time-points was weak, an independent species fingerprinting assay (Automated Ribosomal Intergenic Spacer Analysis) supported this and identified other potentially toxic species. Finally, we assessed population-level genomic variation by mining SNPs from the environmental 2bRAD dataset. Consistent shifts in allele frequencies in P. pungens and P. subpacifica were detected between high and low DA years, suggesting that different intraspecific variants may be associated with prevailing environmental conditions or the presence of DA. Taken together, this method presents a potentially cost-effective and high-throughput approach for studies aiming to evaluate both population and species dynamics in mixed samples. Highlights 2bRAD method facilitates species- and population-level analysis of the same sample Method accurately quantifies species relative abundance with low false positives Consistent shifts in allele frequencies were detected between high and low DA years Certain Pseudo-nitzschia spp. populations may be more associated with DA presence" @default.
• W4229371010 created "2022-05-10" @default.
• W4229371010 creator A5019544749 @default.
• W4229371010 creator A5020900084 @default.
• W4229371010 creator A5040980394 @default.
• W4229371010 creator A5064003180 @default.
• W4229371010 creator A5064867378 @default.
• W4229371010 creator A5067265369 @default.
• W4229371010 creator A5075848516 @default.
• W4229371010 date "2022-05-07" @default.
• W4229371010 modified "2023-09-27" @default.
• W4229371010 title "Reduced representation sequencing accurately quantifies relative abundance and reveals population-level variation in <i>Pseudo-nitzschia</i> spp." @default.
• W4229371010 cites W1532362497 @default.
• W4229371010 cites W185456723 @default.
• W4229371010 cites W1954617363 @default.
• W4229371010 cites W1964920442 @default.
• W4229371010 cites W1967909610 @default.
• W4229371010 cites W1968423560 @default.
• W4229371010 cites W1971303015 @default.
• W4229371010 cites W1971507287 @default.
• W4229371010 cites W1974403393 @default.
• W4229371010 cites W1978555717 @default.
• W4229371010 cites W1981657617 @default.
• W4229371010 cites W1986260377 @default.
• W4229371010 cites W1987191606 @default.
• W4229371010 cites W1988576020 @default.
• W4229371010 cites W2000625831 @default.
• W4229371010 cites W2006654158 @default.
• W4229371010 cites W2016458223 @default.
• W4229371010 cites W2024649694 @default.
• W4229371010 cites W2032143276 @default.
• W4229371010 cites W2033306004 @default.
• W4229371010 cites W2038003976 @default.
• W4229371010 cites W2048924696 @default.
• W4229371010 cites W2067167730 @default.
• W4229371010 cites W2077571765 @default.
• W4229371010 cites W2078851965 @default.
• W4229371010 cites W2092196439 @default.
• W4229371010 cites W2094501562 @default.
• W4229371010 cites W2095489410 @default.
• W4229371010 cites W2105228358 @default.
• W4229371010 cites W2110969687 @default.
• W4229371010 cites W2112906064 @default.
• W4229371010 cites W2114745622 @default.
• W4229371010 cites W2114797031 @default.
• W4229371010 cites W2118166105 @default.
• W4229371010 cites W2121667031 @default.
• W4229371010 cites W2123247627 @default.
• W4229371010 cites W2129628333 @default.
• W4229371010 cites W2131373051 @default.
• W4229371010 cites W2133397972 @default.
• W4229371010 cites W2139173136 @default.
• W4229371010 cites W2143318680 @default.
• W4229371010 cites W2148374146 @default.
• W4229371010 cites W2153349671 @default.
• W4229371010 cites W2156125289 @default.
• W4229371010 cites W2159942570 @default.
• W4229371010 cites W2160219285 @default.
• W4229371010 cites W2160955376 @default.
• W4229371010 cites W2161950199 @default.
• W4229371010 cites W2164198077 @default.
• W4229371010 cites W2169583837 @default.
• W4229371010 cites W2170170241 @default.
• W4229371010 cites W2170551349 @default.
• W4229371010 cites W2170747616 @default.
• W4229371010 cites W2227501310 @default.
• W4229371010 cites W2269040222 @default.
• W4229371010 cites W2315879079 @default.
• W4229371010 cites W2327109388 @default.
• W4229371010 cites W2329529435 @default.
• W4229371010 cites W2521848664 @default.
• W4229371010 cites W2552907226 @default.
• W4229371010 cites W2588329121 @default.
• W4229371010 cites W2620713670 @default.
• W4229371010 cites W2736456658 @default.
• W4229371010 cites W2770405329 @default.
• W4229371010 cites W2778481363 @default.
• W4229371010 cites W2793440556 @default.
• W4229371010 cites W2887638479 @default.
• W4229371010 cites W2898118087 @default.
• W4229371010 cites W2950489962 @default.
• W4229371010 cites W2963199720 @default.
• W4229371010 cites W2969275047 @default.
• W4229371010 cites W2978756410 @default.
• W4229371010 cites W3026753934 @default.
• W4229371010 cites W3039690469 @default.
• W4229371010 cites W3048090444 @default.
• W4229371010 cites W3118971253 @default.
• W4229371010 cites W4236459334 @default.
• W4229371010 cites W75081015 @default.
• W4229371010 cites W938316301 @default.
• W4229371010 doi "https://doi.org/10.1101/2022.05.06.490957" @default.
• W4229371010 hasPublicationYear "2022" @default.
• W4229371010 type Work @default.
• W4229371010 citedByCount "0" @default.
• W4229371010 crossrefType "posted-content" @default.
• W4229371010 hasAuthorship W4229371010A5019544749 @default.
• W4229371010 hasAuthorship W4229371010A5020900084 @default.
• W4229371010 hasAuthorship W4229371010A5040980394 @default.
• W4229371010 hasAuthorship W4229371010A5064003180 @default.

• next