Matches in SemOpenAlex for { <https://semopenalex.org/work/W4229442015> ?p ?o ?g. }
Showing items 1 to 68 of
68
with 100 items per page.
- W4229442015 abstract "<b>Background:</b> Sputum, nasal and bronchial biopsies, and serum of asthmatics have elevated levels of IL17 compared to healthy controls. IL17A is likely to contribute to reduced corticosteroid sensitivity of human airway epithelium. Here, we aimed to investigate the mechanistic underpinnings of this reduced sensitivity in more detail. <b>Methods:</b> Primary human airway epithelial cells (hAECs) were differentiated at an air liquid interface (ALI) and exposed to IL17A (10 ng/ml) in the absence or presence of dexamethasone (Dex, 10 nM) for 14 days. Cells were then collected for RNA sequencing analysis or used for barrier function experiments. Mucus was collected for volume measurement and basal medium for cytokine analysis. <b>Results:</b> 2861 genes were differentially expressed by IL17A (Padj<0.05), of which 2184 genes (92.78%) were not sensitive to Dex (<50% inhibition). Moreover, IL17A inhibited induction of canonical steroid genes by Dex, such as HSD11B2 and FKBP5 (p<0.05). Inflammatory and goblet cell metaplasia markers, cytokine secretion and mucus production were all induced by IL17A, and these effects were not prevented by Dex. However, Dex reversed IL17A-stimulated epithelial disruption (p<0.05) and this was associated with gene expression changes related to cilia function and development. <b>Conclusions:</b> IL17A induces function-specific steroid-insensitivity. Whereas inflammatory response genes and mucus production in primary hAECs in response to IL-17A were steroid-insensitive, corticosteroids were able to reverse IL17A-induced epithelial barrier disruption. The results highlights the function-specific role of corticosteroid-insensitivity in airway epithelial cells." @default.
- W4229442015 created "2022-05-11" @default.
- W4229442015 creator A5007054215 @default.
- W4229442015 creator A5019929850 @default.
- W4229442015 creator A5033349957 @default.
- W4229442015 creator A5060340541 @default.
- W4229442015 creator A5080412024 @default.
- W4229442015 date "2022-03-10" @default.
- W4229442015 modified "2023-09-29" @default.
- W4229442015 title "Function-specific IL17A and Dexamethasone interactions in primary human airway epithelial cells" @default.
- W4229442015 doi "https://doi.org/10.1183/23120541.lsc-2022.150" @default.
- W4229442015 hasPublicationYear "2022" @default.
- W4229442015 type Work @default.
- W4229442015 citedByCount "0" @default.
- W4229442015 crossrefType "proceedings-article" @default.
- W4229442015 hasAuthorship W4229442015A5007054215 @default.
- W4229442015 hasAuthorship W4229442015A5019929850 @default.
- W4229442015 hasAuthorship W4229442015A5033349957 @default.
- W4229442015 hasAuthorship W4229442015A5060340541 @default.
- W4229442015 hasAuthorship W4229442015A5080412024 @default.
- W4229442015 hasConcept C134018914 @default.
- W4229442015 hasConcept C1491633281 @default.
- W4229442015 hasConcept C18903297 @default.
- W4229442015 hasConcept C203014093 @default.
- W4229442015 hasConcept C2777312293 @default.
- W4229442015 hasConcept C2778690821 @default.
- W4229442015 hasConcept C2780401358 @default.
- W4229442015 hasConcept C49039625 @default.
- W4229442015 hasConcept C51911345 @default.
- W4229442015 hasConcept C529295009 @default.
- W4229442015 hasConcept C54355233 @default.
- W4229442015 hasConcept C55493867 @default.
- W4229442015 hasConcept C81729549 @default.
- W4229442015 hasConcept C86803240 @default.
- W4229442015 hasConcept C87568996 @default.
- W4229442015 hasConcept C95444343 @default.
- W4229442015 hasConceptScore W4229442015C134018914 @default.
- W4229442015 hasConceptScore W4229442015C1491633281 @default.
- W4229442015 hasConceptScore W4229442015C18903297 @default.
- W4229442015 hasConceptScore W4229442015C203014093 @default.
- W4229442015 hasConceptScore W4229442015C2777312293 @default.
- W4229442015 hasConceptScore W4229442015C2778690821 @default.
- W4229442015 hasConceptScore W4229442015C2780401358 @default.
- W4229442015 hasConceptScore W4229442015C49039625 @default.
- W4229442015 hasConceptScore W4229442015C51911345 @default.
- W4229442015 hasConceptScore W4229442015C529295009 @default.
- W4229442015 hasConceptScore W4229442015C54355233 @default.
- W4229442015 hasConceptScore W4229442015C55493867 @default.
- W4229442015 hasConceptScore W4229442015C81729549 @default.
- W4229442015 hasConceptScore W4229442015C86803240 @default.
- W4229442015 hasConceptScore W4229442015C87568996 @default.
- W4229442015 hasConceptScore W4229442015C95444343 @default.
- W4229442015 hasLocation W42294420151 @default.
- W4229442015 hasOpenAccess W4229442015 @default.
- W4229442015 hasPrimaryLocation W42294420151 @default.
- W4229442015 hasRelatedWork W1939566065 @default.
- W4229442015 hasRelatedWork W1982930427 @default.
- W4229442015 hasRelatedWork W2070204844 @default.
- W4229442015 hasRelatedWork W2395694889 @default.
- W4229442015 hasRelatedWork W2414217840 @default.
- W4229442015 hasRelatedWork W2905868380 @default.
- W4229442015 hasRelatedWork W2919734783 @default.
- W4229442015 hasRelatedWork W2954640148 @default.
- W4229442015 hasRelatedWork W3030950285 @default.
- W4229442015 hasRelatedWork W4283723740 @default.
- W4229442015 isParatext "false" @default.
- W4229442015 isRetracted "false" @default.
- W4229442015 workType "article" @default.