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• W4229444595 abstract "Abstract The epidemiological association between exposure to particulate matter (PM 10 ) and various respiratory and cardiovascular problems is well known, but the mechanisms driving these effects remain unclear. Neutrophils play an essential role in immune defense against foreign agents and also participate in the development of inflammatory responses. However, the role of these cells in the PM 10 induced inflammatory response is not yet fully established. Thus, this study aims to evaluate the effect of PM 10 on the neutrophil-mediated inflammatory response. For this, neutrophils from healthy adult human donors were in vitro exposed to different concentrations of PM 10 . The cell viability and cytotoxic activity were evaluated by MTT. LDH, propidium iodide and reactive oxygen species (ROS) were quantified by flow cytometry. Interleukin 8 (IL-8) expression, peptidyl arginine deiminase 4 (PAD 4 ), myeloperoxidase (MPO), and neutrophil elastase (NE) expression were measured by RT-PCR. IL-8 was also quantified by ELISA. Fluorescence microscopy was used to evaluate neutrophil extracellular traps (NETs) release. The in vivo inflammatory responses were assessed in BALB/c mice exposed to PM 10 by histopathology and RT-PCR. The analysis shows that PM 10 exposure induced a cytotoxic effect on neutrophils, evidenced by necrosis and LDH release at high PM 10 concentrations. ROS production, IL-8, MPO, NE expression, and NETs release were increased at all PM 10 concentrations assessed. Neutrophil infiltration in bronchoalveolar lavage fluid (BALF), histopathological changes with inflammatory cell infiltration, and CXCL1 expression were observed in PM 10 -treated mice. The results suggest that lung inflammation in response to PM 10 could be mediated by neutrophils activation. In this case, these cells migrate to the lungs and release pro-inflamatory mediators, including ROS, IL-8, and NETs. Thus, contributing to the exacerbation of respiratory pathologies, such as allergies, infectious and obstructive diseases." @default.
• W4229444595 created "2022-05-11" @default.
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• W4229444595 date "2022-05-09" @default.
• W4229444595 modified "2023-10-18" @default.
• W4229444595 title "Particulate matter (PM10) induces in vitro activation of human neutrophils, and lung histopathological alterations in a mouse model" @default.
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• W4229444595 cites W1968739991 @default.
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• W4229444595 cites W1977004810 @default.
• W4229444595 cites W2011340228 @default.
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• W4229444595 cites W2025656009 @default.
• W4229444595 cites W2025694904 @default.
• W4229444595 cites W2029628372 @default.
• W4229444595 cites W2046478419 @default.
• W4229444595 cites W2050456214 @default.
• W4229444595 cites W2059344814 @default.
• W4229444595 cites W2060463620 @default.
• W4229444595 cites W2066569321 @default.
• W4229444595 cites W2070165558 @default.
• W4229444595 cites W2073092560 @default.
• W4229444595 cites W2093161332 @default.
• W4229444595 cites W2115772647 @default.
• W4229444595 cites W2118748012 @default.
• W4229444595 cites W2144873766 @default.
• W4229444595 cites W2156895145 @default.
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• W4229444595 cites W2159885030 @default.
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• W4229444595 cites W2283708611 @default.
• W4229444595 cites W2301131921 @default.
• W4229444595 cites W2308360335 @default.
• W4229444595 cites W2412276111 @default.
• W4229444595 cites W2461611841 @default.
• W4229444595 cites W2473153801 @default.
• W4229444595 cites W2579972680 @default.
• W4229444595 cites W2606913425 @default.
• W4229444595 cites W2738355493 @default.
• W4229444595 cites W2747072323 @default.
• W4229444595 cites W2762816177 @default.
• W4229444595 cites W2789997601 @default.
• W4229444595 cites W2792025613 @default.
• W4229444595 cites W2864532440 @default.
• W4229444595 cites W2877716630 @default.
• W4229444595 cites W2888081476 @default.
• W4229444595 cites W2889238980 @default.
• W4229444595 cites W2894561194 @default.
• W4229444595 cites W2899751377 @default.
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• W4229444595 cites W2983203594 @default.
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• W4229444595 cites W2991048273 @default.
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• W4229444595 cites W2997836999 @default.
• W4229444595 cites W3005699493 @default.
• W4229444595 cites W3007014416 @default.
• W4229444595 cites W3015332015 @default.
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• W4229444595 cites W3037020655 @default.
• W4229444595 cites W3037284374 @default.
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• W4229444595 doi "https://doi.org/10.1038/s41598-022-11553-6" @default.
• W4229444595 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35534522" @default.
• W4229444595 hasPublicationYear "2022" @default.
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