FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4229453617> ?p ?o ?g. }

• W4229453617 abstract "The yeast Ogataea polymorpha is an upcoming host for bio-manufacturing due to its unique physiological properties, including its broad substrate spectrum, and particularly its ability to utilize methanol as the sole carbon and energy source. However, metabolic engineering tools for O. polymorpha are still rare. In this study we characterized the influence of 6 promoters and 15 terminators on gene expression throughout batch cultivations with glucose, glycerol, and methanol as carbon sources as well as mixes of these carbon sources. For this characterization, a short half-life Green Fluorescent Protein (GFP) variant was chosen, which allows a precise temporal resolution of gene expression. Our promoter studies revealed how different promoters do not only influence the expression strength but also the timepoint of maximal expression. For example, the expression strength of the catalase promoter (pCAT) and the methanol oxidase promoter (pMOX) are comparable on methanol, but the maximum expression level of the pCAT is reached more than 24 h earlier. By varying the terminators, a 6-fold difference in gene expression was achieved with the MOX terminator boosting gene expression on all carbon sources by around 50% compared to the second-strongest terminator. It was shown that this exceptional increase in gene expression is achieved by the MOX terminator stabilizing the mRNA, which results in an increased transcript level in the cells. We further found that different pairing of promoters and terminators or the expression of a different gene (β-galactosidase gene) did not influence the performance of the genetic parts. Consequently, it is possible to mix and match promoters and terminators as independent elements to tune gene expression in O. polymorpha." @default.
• W4229453617 created "2022-05-11" @default.
• W4229453617 creator A5040665605 @default.
• W4229453617 creator A5046799748 @default.
• W4229453617 creator A5088567403 @default.
• W4229453617 creator A5089183823 @default.
• W4229453617 date "2022-05-10" @default.
• W4229453617 modified "2023-09-30" @default.
• W4229453617 title "Mix and Match: Promoters and Terminators for Tuning Gene Expression in the Methylotrophic Yeast Ogataea polymorpha" @default.
• W4229453617 cites W1861787140 @default.
• W4229453617 cites W1862509991 @default.
• W4229453617 cites W1873692790 @default.
• W4229453617 cites W1971933513 @default.
• W4229453617 cites W1981898996 @default.
• W4229453617 cites W1995931397 @default.
• W4229453617 cites W2003676968 @default.
• W4229453617 cites W2007405375 @default.
• W4229453617 cites W2010835482 @default.
• W4229453617 cites W2017608196 @default.
• W4229453617 cites W2019815305 @default.
• W4229453617 cites W2043382556 @default.
• W4229453617 cites W2056349974 @default.
• W4229453617 cites W2076922386 @default.
• W4229453617 cites W2077347075 @default.
• W4229453617 cites W2082020530 @default.
• W4229453617 cites W2082658854 @default.
• W4229453617 cites W2086561953 @default.
• W4229453617 cites W2099521715 @default.
• W4229453617 cites W2101442503 @default.
• W4229453617 cites W2106430658 @default.
• W4229453617 cites W2107185990 @default.
• W4229453617 cites W2107277218 @default.
• W4229453617 cites W2116253414 @default.
• W4229453617 cites W2127398938 @default.
• W4229453617 cites W2131548424 @default.
• W4229453617 cites W2133238709 @default.
• W4229453617 cites W2137076128 @default.
• W4229453617 cites W2143488845 @default.
• W4229453617 cites W2149079511 @default.
• W4229453617 cites W2151546588 @default.
• W4229453617 cites W2168695178 @default.
• W4229453617 cites W2192080449 @default.
• W4229453617 cites W2213454576 @default.
• W4229453617 cites W2313226053 @default.
• W4229453617 cites W2375179107 @default.
• W4229453617 cites W2512706593 @default.
• W4229453617 cites W2514281930 @default.
• W4229453617 cites W2559716871 @default.
• W4229453617 cites W2560849915 @default.
• W4229453617 cites W2654116011 @default.
• W4229453617 cites W2765391112 @default.
• W4229453617 cites W2775688428 @default.
• W4229453617 cites W2790533646 @default.
• W4229453617 cites W2791815430 @default.
• W4229453617 cites W2810352238 @default.
• W4229453617 cites W2897677281 @default.
• W4229453617 cites W2943065770 @default.
• W4229453617 cites W2945072622 @default.
• W4229453617 cites W2983215973 @default.
• W4229453617 cites W3045322230 @default.
• W4229453617 cites W3090187112 @default.
• W4229453617 cites W3107218515 @default.
• W4229453617 cites W3127099707 @default.
• W4229453617 cites W4243853204 @default.
• W4229453617 cites W84074421 @default.
• W4229453617 cites W3212662576 @default.
• W4229453617 doi "https://doi.org/10.3389/fbioe.2022.876316" @default.
• W4229453617 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/35620471" @default.
• W4229453617 hasPublicationYear "2022" @default.
• W4229453617 type Work @default.
• W4229453617 citedByCount "4" @default.
• W4229453617 countsByYear W42294536172022 @default.
• W4229453617 countsByYear W42294536172023 @default.
• W4229453617 crossrefType "journal-article" @default.
• W4229453617 hasAuthorship W4229453617A5040665605 @default.
• W4229453617 hasAuthorship W4229453617A5046799748 @default.
• W4229453617 hasAuthorship W4229453617A5088567403 @default.
• W4229453617 hasAuthorship W4229453617A5089183823 @default.
• W4229453617 hasBestOaLocation W42294536171 @default.
• W4229453617 hasConcept C100012643 @default.
• W4229453617 hasConcept C101762097 @default.
• W4229453617 hasConcept C104317684 @default.
• W4229453617 hasConcept C108305142 @default.
• W4229453617 hasConcept C116403925 @default.
• W4229453617 hasConcept C121332964 @default.
• W4229453617 hasConcept C1276947 @default.
• W4229453617 hasConcept C150194340 @default.
• W4229453617 hasConcept C153911025 @default.
• W4229453617 hasConcept C165864922 @default.
• W4229453617 hasConcept C178046730 @default.
• W4229453617 hasConcept C2779222958 @default.
• W4229453617 hasConcept C40767141 @default.
• W4229453617 hasConcept C54355233 @default.
• W4229453617 hasConcept C86803240 @default.
• W4229453617 hasConcept C92087593 @default.
• W4229453617 hasConceptScore W4229453617C100012643 @default.
• W4229453617 hasConceptScore W4229453617C101762097 @default.
• W4229453617 hasConceptScore W4229453617C104317684 @default.
• W4229453617 hasConceptScore W4229453617C108305142 @default.
• W4229453617 hasConceptScore W4229453617C116403925 @default.

• next