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- W4229560774 abstract "Abstract. A heterologous immunoassay for 2-hydroxyoestrogens 1 has been established in which antibodies raised against 2-hydroxyoestradiol-17-succinyl-BSA serve as binding protein and 2-hydroxyoestrone-17-cmo-[ 125 I]iodohistamine as radioligand. Lipophilic serum components competing for binding sites in this system were defined as 'total 2-hydroxyoestrogens'. The underlying assumption of specificity was supported by the pattern of cross-reactivity evaluated with structural related steroids and o-diphenols and by the fact, that an additional chromatography of the serum extracts preceding the competing reaction had little if any effect. Sensitivity: 2.8 ± 1 pg/tube; accuracy: Y = 0.91x + 2.2; r = 0.989; precision: 5.8% intra-assay; 6.5% inter-assay. The following concentrations ( ± standard deviation) were found in the sera of healthy subjects. Young men: 29 ± 5 pg/ml (n = 11); women follicular phase: 32 ± 8 pg/ml (n = 25); luteal phase: 53 ± 13 pg/ml (n = 23); postmenopausal women: 13 ± 4 pg/ml (n = 10); pregnant women 11th–20th week: 70 ± 16 mg/ml (n = 64); 36th–40th week: 240 ± 23 pg/ml (n = 40); newborn cord blood: 604 ± 43 pg/ml (n = 48)." @default.
- W4229560774 created "2022-05-11" @default.
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- W4229560774 date "1982-05-01" @default.
- W4229560774 modified "2023-09-30" @default.
- W4229560774 title "Concentrations of 2-hydroxyoestrogens in human sera measured by a heterologous immunoassay with an 125I-labelled ligand" @default.
- W4229560774 doi "https://doi.org/10.1530/acta.0.1000154" @default.
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