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- W4229589038 abstract "A rapid method for preparing cell-free extracts of Aspergillus ochraceus was developed. Mycelial mats were prefrozen in liquid nitrogen, ground to a fine powder in a cold mortar, and homogenized in an all-glass mechanical homogenizer. This method provided preparations averaging 43.0 mg of protein per g of mycelium (wet weight). The method was fast, efficient, and did not subject the extract to temperatures above 1 C or to heavy metals. The preparation method was suitable for studying a variety of in vitro fungal enzyme systems. Amylase, acid phosphatase, alkaline phosphatase, catalase, fatty acid synthetase, glucose-6-phosphate dehydrogenase, beta-glucosidase, beta fructofuranosidase, and trehalase activities were measurable in the preparations." @default.
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- W4229589038 date "1975-01-01" @default.
- W4229589038 modified "2023-09-26" @default.
- W4229589038 title "Rapid Method for Preparing Cell-Free Extracts of Aspergillus ochraceus" @default.
- W4229589038 doi "https://doi.org/10.1128/aem.29.2.300-302.1975" @default.
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