Matches in SemOpenAlex for { <https://semopenalex.org/work/W4229675802> ?p ?o ?g. }
- W4229675802 endingPage "4203" @default.
- W4229675802 startingPage "4194" @default.
- W4229675802 abstract "Laminins are a family of disulfide-linked heterotrimeric proteins consisting of 3 different subunits termed α, β, and γ chains. Combinations of 11 characterized laminin subunits (α1-α5, β1-β3, and γ1-γ3) generate at least 12 laminin isoforms, which can serve different functions. Although expression of laminin in the hematopoietic microenvironment has been known for many years, the nature of the laminin isoforms present in the human bone marrow is poorly characterized. The present study attempts to clarify this issue. Reverse transcriptase–polymerase chain reaction analysis of human bone marrow stromal cells suggested the expression of many laminin isoforms in the marrow. Northern blot and immunoblot analysis, however, showed that laminin-8/9 and laminin-10/11 are the most abundant laminin isoforms synthesized by human bone marrow stromal cells. Other isoforms, if present, certainly play a minor role in the hematopoietic microenvironment. Functionally, laminin-10/11 preparations showed strong adhesive interactions with human CD34+ cell lines. Antibodies against the β1 integrin subunit inhibited these interactions. Other laminin isoforms, especially laminin-1 and laminin-2/4, showed only weak or no adhesive interactions with the hematopoietic cell lines tested, explaining former negative results. In addition to its adhesion-mediating properties, laminin-10/11 preparations also showed a mitogenic activity for human hematopoietic progenitor cells. Taken together, these data suggest that laminin in the bone marrow plays a hitherto unexpected important function in the development of hematopoietic progenitor cells." @default.
- W4229675802 created "2022-05-11" @default.
- W4229675802 creator A5002532057 @default.
- W4229675802 creator A5008632674 @default.
- W4229675802 creator A5031719922 @default.
- W4229675802 creator A5032260421 @default.
- W4229675802 creator A5036494601 @default.
- W4229675802 creator A5062918646 @default.
- W4229675802 creator A5083183929 @default.
- W4229675802 creator A5085385520 @default.
- W4229675802 date "2000-12-15" @default.
- W4229675802 modified "2023-10-18" @default.
- W4229675802 title "Characterization and functional analysis of laminin isoforms in human bone marrow" @default.
- W4229675802 cites W128841889 @default.
- W4229675802 cites W1515102140 @default.
- W4229675802 cites W1542620420 @default.
- W4229675802 cites W1567770297 @default.
- W4229675802 cites W1878446582 @default.
- W4229675802 cites W191322410 @default.
- W4229675802 cites W1924777753 @default.
- W4229675802 cites W1933015809 @default.
- W4229675802 cites W1951540826 @default.
- W4229675802 cites W1967323327 @default.
- W4229675802 cites W1967828409 @default.
- W4229675802 cites W1973100852 @default.
- W4229675802 cites W1974262404 @default.
- W4229675802 cites W1975986023 @default.
- W4229675802 cites W1978812188 @default.
- W4229675802 cites W1981269581 @default.
- W4229675802 cites W1988470533 @default.
- W4229675802 cites W1989768887 @default.
- W4229675802 cites W2001991657 @default.
- W4229675802 cites W2018042479 @default.
- W4229675802 cites W2018184596 @default.
- W4229675802 cites W2040413836 @default.
- W4229675802 cites W2041262306 @default.
- W4229675802 cites W2052728159 @default.
- W4229675802 cites W2058809631 @default.
- W4229675802 cites W2059766176 @default.
- W4229675802 cites W2075147221 @default.
- W4229675802 cites W2080327002 @default.
- W4229675802 cites W2092044791 @default.
- W4229675802 cites W2093514870 @default.
- W4229675802 cites W2107564206 @default.
- W4229675802 cites W2111069558 @default.
- W4229675802 cites W2111786954 @default.
- W4229675802 cites W2119622097 @default.
- W4229675802 cites W2122989956 @default.
- W4229675802 cites W2125197879 @default.
- W4229675802 cites W2132194828 @default.
- W4229675802 cites W2138919048 @default.
- W4229675802 cites W2140292861 @default.
- W4229675802 cites W2143987989 @default.
- W4229675802 cites W2152284637 @default.
- W4229675802 cites W2165123145 @default.
- W4229675802 cites W2169342001 @default.
- W4229675802 cites W2169968306 @default.
- W4229675802 cites W2177231732 @default.
- W4229675802 cites W2200231061 @default.
- W4229675802 cites W2323367363 @default.
- W4229675802 cites W2410369169 @default.
- W4229675802 cites W341993295 @default.
- W4229675802 cites W4230347328 @default.
- W4229675802 cites W4241680484 @default.
- W4229675802 cites W4247546883 @default.
- W4229675802 cites W4294216491 @default.
- W4229675802 doi "https://doi.org/10.1182/blood.v96.13.4194.h8004194_4194_4203" @default.
- W4229675802 hasPublicationYear "2000" @default.
- W4229675802 type Work @default.
- W4229675802 citedByCount "4" @default.
- W4229675802 countsByYear W42296758022021 @default.
- W4229675802 countsByYear W42296758022023 @default.
- W4229675802 crossrefType "journal-article" @default.
- W4229675802 hasAuthorship W4229675802A5002532057 @default.
- W4229675802 hasAuthorship W4229675802A5008632674 @default.
- W4229675802 hasAuthorship W4229675802A5031719922 @default.
- W4229675802 hasAuthorship W4229675802A5032260421 @default.
- W4229675802 hasAuthorship W4229675802A5036494601 @default.
- W4229675802 hasAuthorship W4229675802A5062918646 @default.
- W4229675802 hasAuthorship W4229675802A5083183929 @default.
- W4229675802 hasAuthorship W4229675802A5085385520 @default.
- W4229675802 hasConcept C104317684 @default.
- W4229675802 hasConcept C109159458 @default.
- W4229675802 hasConcept C1491633281 @default.
- W4229675802 hasConcept C153911025 @default.
- W4229675802 hasConcept C16930146 @default.
- W4229675802 hasConcept C189165786 @default.
- W4229675802 hasConcept C195687474 @default.
- W4229675802 hasConcept C201750760 @default.
- W4229675802 hasConcept C203014093 @default.
- W4229675802 hasConcept C2779814568 @default.
- W4229675802 hasConcept C2780007613 @default.
- W4229675802 hasConcept C28328180 @default.
- W4229675802 hasConcept C502942594 @default.
- W4229675802 hasConcept C53345823 @default.
- W4229675802 hasConcept C55493867 @default.
- W4229675802 hasConcept C86803240 @default.
- W4229675802 hasConcept C95444343 @default.