Matches in SemOpenAlex for { <https://semopenalex.org/work/W4229692756> ?p ?o ?g. }
- W4229692756 endingPage "7944" @default.
- W4229692756 startingPage "7938" @default.
- W4229692756 abstract "Abstract The pre‐targeted imaging of enzyme activity has not been reported, likely owing to the lack of a mechanism to retain the injected substrate in the first step for subsequent labeling. Herein, we report the use of two bioorthogonal reactions—the condensation reaction of aromatic nitriles and aminothiols and the inverse‐electron demand Diels–Alder reaction between tetrazine and trans ‐cyclooctene (TCO)—to develop a novel strategy for pre‐targeted imaging of the activity of proteases. The substrate probe ( TCO‐C‐SNAT4 ) can be selectively activated by an enzyme target (e.g. caspase‐3/7), which triggers macrocyclization and subsequent in situ self‐assembly into nanoaggregates retained at the target site. The tetrazine‐imaging tag conjugate labels TCO in the nanoaggregates to generate selective signal retention for imaging in vitro, in cells, and in mice. Owing to the decoupling of enzyme activation and imaging tag immobilization, TCO‐C‐SNAT4 can be repeatedly injected to generate and accumulate more TCO‐nanoaggregates for click labeling." @default.
- W4229692756 created "2022-05-11" @default.
- W4229692756 creator A5012718640 @default.
- W4229692756 creator A5027490097 @default.
- W4229692756 creator A5032092856 @default.
- W4229692756 creator A5073112631 @default.
- W4229692756 date "2020-03-20" @default.
- W4229692756 modified "2023-10-10" @default.
- W4229692756 title "Pre‐targeted Imaging of Protease Activity through In Situ Assembly of Nanoparticles" @default.
- W4229692756 cites W1155937765 @default.
- W4229692756 cites W1533229981 @default.
- W4229692756 cites W1785852384 @default.
- W4229692756 cites W1983667894 @default.
- W4229692756 cites W1989091022 @default.
- W4229692756 cites W1989368359 @default.
- W4229692756 cites W1990765611 @default.
- W4229692756 cites W1991945497 @default.
- W4229692756 cites W1999487949 @default.
- W4229692756 cites W2010214999 @default.
- W4229692756 cites W2010912389 @default.
- W4229692756 cites W2025562232 @default.
- W4229692756 cites W2028768078 @default.
- W4229692756 cites W2033439612 @default.
- W4229692756 cites W2051484569 @default.
- W4229692756 cites W2062524026 @default.
- W4229692756 cites W2065293903 @default.
- W4229692756 cites W2068258715 @default.
- W4229692756 cites W2069977295 @default.
- W4229692756 cites W2075503331 @default.
- W4229692756 cites W2082407523 @default.
- W4229692756 cites W2088497317 @default.
- W4229692756 cites W2099402486 @default.
- W4229692756 cites W2102189935 @default.
- W4229692756 cites W2103272211 @default.
- W4229692756 cites W2103673003 @default.
- W4229692756 cites W2111789849 @default.
- W4229692756 cites W2112175472 @default.
- W4229692756 cites W2112322230 @default.
- W4229692756 cites W2126921829 @default.
- W4229692756 cites W2132257110 @default.
- W4229692756 cites W2134413630 @default.
- W4229692756 cites W2134988306 @default.
- W4229692756 cites W2135303044 @default.
- W4229692756 cites W2136817553 @default.
- W4229692756 cites W2146604108 @default.
- W4229692756 cites W2155827990 @default.
- W4229692756 cites W2175893746 @default.
- W4229692756 cites W2291424992 @default.
- W4229692756 cites W2292719107 @default.
- W4229692756 cites W2332097090 @default.
- W4229692756 cites W2395832305 @default.
- W4229692756 cites W2422217246 @default.
- W4229692756 cites W2443630477 @default.
- W4229692756 cites W2526827711 @default.
- W4229692756 cites W2570731899 @default.
- W4229692756 cites W2725869510 @default.
- W4229692756 cites W2751614520 @default.
- W4229692756 cites W2779122303 @default.
- W4229692756 cites W2800788936 @default.
- W4229692756 cites W2803024865 @default.
- W4229692756 cites W2884049259 @default.
- W4229692756 cites W2996133723 @default.
- W4229692756 cites W4234055631 @default.
- W4229692756 cites W4243035494 @default.
- W4229692756 cites W4243350571 @default.
- W4229692756 cites W4247794123 @default.
- W4229692756 cites W4248003274 @default.
- W4229692756 cites W4248522153 @default.
- W4229692756 cites W4248650850 @default.
- W4229692756 cites W4248853096 @default.
- W4229692756 cites W4253422044 @default.
- W4229692756 cites W4383185065 @default.
- W4229692756 doi "https://doi.org/10.1002/ange.201916352" @default.
- W4229692756 hasPublicationYear "2020" @default.
- W4229692756 type Work @default.
- W4229692756 citedByCount "14" @default.
- W4229692756 countsByYear W42296927562021 @default.
- W4229692756 countsByYear W42296927562022 @default.
- W4229692756 countsByYear W42296927562023 @default.
- W4229692756 crossrefType "journal-article" @default.
- W4229692756 hasAuthorship W4229692756A5012718640 @default.
- W4229692756 hasAuthorship W4229692756A5027490097 @default.
- W4229692756 hasAuthorship W4229692756A5032092856 @default.
- W4229692756 hasAuthorship W4229692756A5073112631 @default.
- W4229692756 hasConcept C111368507 @default.
- W4229692756 hasConcept C12554922 @default.
- W4229692756 hasConcept C127313418 @default.
- W4229692756 hasConcept C134306372 @default.
- W4229692756 hasConcept C171250308 @default.
- W4229692756 hasConcept C178790620 @default.
- W4229692756 hasConcept C181199279 @default.
- W4229692756 hasConcept C185592680 @default.
- W4229692756 hasConcept C192562407 @default.
- W4229692756 hasConcept C197336794 @default.
- W4229692756 hasConcept C21951064 @default.
- W4229692756 hasConcept C2776714187 @default.
- W4229692756 hasConcept C2777289219 @default.
- W4229692756 hasConcept C2777822432 @default.