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- W4229745186 abstract "Current Protocols in PharmacologyVolume 87, Issue 1 e53 ISSUE INFORMATIONFree Access Issue Information TOC First published: 22 December 2019 https://doi.org/10.1002/cpph.53AboutPDF ToolsRequest permissionExport citationAdd to favoritesTrack citation ShareShare Give accessShare full text accessShare full-text accessPlease review our Terms and Conditions of Use and check box below to share full-text version of article.I have read and accept the Wiley Online Library Terms and Conditions of UseShareable LinkUse the link below to share a full-text version of this article with your friends and colleagues. Learn more.Copy URL Share a linkShare onFacebookTwitterLinkedInRedditWechat Graphical Abstract Cover: In Liu et al. (https://doi.org/10.1002/cpph.69), the image shows a comparison between conventional manual patch clamp and automated electrophysiology. (A) Schematic of manual patch clamp recording (left panel) under whole-cell configuration. A single cell is recorded using a glass recording pipette. Compounds can be applied to the extracellular solution (blue), whereas the artificial intracellular solution (green) is contained in the pipette. Right panel: voltage pulse protocol and representative transient inward Nav1.7 channel current. (B) Schematic of planar array–based automated electrophysiology experiment. A single cell is recorded through a micropore in a single well (upper left) from a chip containing 384 wells (upper right). Both extracellular (blue) and intracellular (green) solutions can be changed by perfusion. Lower panel: representative result from one Syncropatch run. Wells were color coded based on seal resistance. Resistance, cells: green, >0.5 GΩ, 350/384; blue, 100 to 500 MΩ, 30/384; white, <100 MΩ, 4/384. Current traces within each well were autoscaled for maximum display. View the latest in Pharmacology RelatedInformation" @default.
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- W4229745186 date "2019-12-01" @default.
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