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• W4230203249 abstract "Abstract Background Molecular detection of low-density Plasmodium falciparum infections is essential for surveillance studies conducted to inform malaria control strategies in close-to-elimination settings. Molecular monitoring of residual malaria infections usually requires a large study size, therefore sampling and diagnostic processes need to be economical and optimized for high-throughput. In a method comparison, we aimed at identifying the most efficient diagnostic procedure for processing large collections of community samples with optimal test sensitivity, simplicity, and minimal costs.Methods In a reactive case detection study conducted on Zanzibar, parasitemia of 4590 individuals of all ages was investigated by a highly sensitive quantitative (q) PCR that targets multiple var gene copies per parasite genome. To reduce cost, a first round of positivity screening was performed on pools of dried blood spots from five individuals. Ten cycles of a pre-PCR were performed directly on the filter paper punches, followed by qPCR. In a second round, samples of positive pools were individually analyzed by pre-PCR and qPCR.Results Prevalence in household members and neighbors of index cases was 1.7% (78/4590) with a geometric mean parasite density of 58 parasites/µl blood. Using qPCR as gold standard, diagnostic sensitivity of rapid diagnostic tests (RDTs) was 37% (29/78). Infections positive by qPCR but negative by RDT had mean densities of 15 parasites/µl blood. Conclusion The approach of pre-screening reactive case detection samples in pools of five was ideal for a low prevalence setting such as in Zanzibar. Performing direct PCR on filter paper punches saves substantial time and justifies the higher cost for a polymerase suitable for amplifying DNA directly from whole blood. Molecular monitoring in community samples provided a more accurate picture of infection prevalence compared to RDT results alone, by identifying a reservoir of infection which is largely missed by RDT. qPCR as a research and surveillance tool can great benefits the evaluation of strategies or programs to eliminate malaria." @default.
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• W4230203249 date "2019-11-25" @default.
• W4230203249 modified "2023-09-27" @default.
• W4230203249 title "Molecular methods for tracking residual P. falciparum transmission in a close-to-elimination setting in Zanzibar" @default.
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• W4230203249 doi "https://doi.org/10.21203/rs.2.17710/v1" @default.
• W4230203249 hasPublicationYear "2019" @default.
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