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• W4230222434 endingPage "293" @default.
• W4230222434 startingPage "281" @default.
• W4230222434 abstract "Protection experiments with antibodies against small nuclear ribonucleoproteins (snRNPs) have elucidated the location of and requirements for interactions between snRNPs and human beta-globin transcripts during splicing in vitro. U2 snRNP association with the intron branch site continues after branch formation, requires intact U2 RNA, and is affected by some alterations of the 3' splice site sequence. U2 snRNP binding to the branched intermediate and U1 snRNP protection of an extended 5' splice region are detected exclusively in spliceosome fractions, indicating that both snRNPs are spliceosome components. While each snRNP associates specifically with the pre-mRNA, they also appear to interact with each other. The recovery of fragments mapping upstream of the 5' splice site suggests how the excised exon is held in the spliceosome." @default.
• W4230222434 created "2022-05-11" @default.
• W4230222434 creator A5029580614 @default.
• W4230222434 creator A5035064101 @default.
• W4230222434 date "1987-01-01" @default.
• W4230222434 modified "2023-09-29" @default.
• W4230222434 title "Multiple interactions between the splicing substrate and small nuclear ribonucleoproteins in spliceosomes" @default.
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• W4230222434 doi "https://doi.org/10.1128/mcb.7.1.281-293.1987" @default.
• W4230222434 hasPublicationYear "1987" @default.
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