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- W4230311266 abstract "This chapter is intended to help the reader understand the influence of processing procedures on the health of cells prior to cryopreservation and strategies to mitigate stress on the cells. Two different types of stress result from pre-freeze process, includes hypoxia resulting from cutting off the blood supply to the tissue and stress associated with enzymatic digestion of the tissue. Pre-freeze processing may also result in contamination of the cells with adventitious agents, which include viruses, bacteria, mycoplasma, fungi, rickettsia, protozoa, parasites, and transmissible spongiform encephalitis agents. The cells are monitored during processing for early signs of apoptosis or shifts in metabolism as markers for sublethal stressing of the cells. Cell characterization is a critical step in preservation processing. Pre-freeze characterization includes determination of the following: dentity, genetic stability, enumeration, purity and adventitious infectious agents. Commercial testing laboratories have standard batteries of tests designed to screen for these agents." @default.
- W4230311266 created "2022-05-11" @default.
- W4230311266 date "2017-12-01" @default.
- W4230311266 modified "2023-09-23" @default.
- W4230311266 title "Pre-freeze Processing and Characterization" @default.
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- W4230311266 doi "https://doi.org/10.1002/9781118989883.ch2" @default.
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