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- W4230347367 abstract "The in vitro assay developed by Hartmann and Ledergerber (1995)[1] utilizing the Spectrofluorimetric quantification of malondialdehyde after reaction with thiobarbituric acid was modified and used for further investigations. The human whole blood was replaced by a platelet suspension of pig blood, and calcium ionophore A23187 was used instead of collagen for inducing the arachidonic acid cascade. The modified assay represents a simple, time and cost saving method for the evaluation of cyclooxygenase-1/thromboxane synthase inhibition. The reproducibility and comparability of results is given. Additional experiments allow classification of selective phospholipase A2, cyclooxygenase-1, and thromboxane synthase inhibitors. Further studies of malondialdehyde formation show that the cyclooxygenase and/or the thromboxane synthase are competitively inhibited by reaction products of the cyclooxygenase pathway by a negative feedback mechanism." @default.
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- W4230347367 date "1998-11-01" @default.
- W4230347367 modified "2023-10-18" @default.
- W4230347367 title "Spectrofluorimetric Quantification of Malondialdehyde for Evaluation of Cyclooxygenase-1/Thromboxane Synthase Inhibition" @default.
- W4230347367 doi "https://doi.org/10.1002/(sici)1521-4184(199811)331:11<359::aid-ardp359>3.3.co;2-2" @default.
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