Matches in SemOpenAlex for { <https://semopenalex.org/work/W4230377260> ?p ?o ?g. }
Showing items 1 to 72 of
72
with 100 items per page.
- W4230377260 endingPage "4261" @default.
- W4230377260 startingPage "4251" @default.
- W4230377260 abstract "Direct examination of the interacting sites between PTH and the human PTH2 receptor (PTH2R) was conducted by photoaffinity cross-linking followed by protein digestion and mapping of the radiolabeled photoconjugated receptor. Photoreactive analogs of PTH, individually substituted with an l-p-benzoylphenylalanine (Bpa) at each of the first 6 N-terminal positions, were pharmacologically evaluated in cells stably expressing recombinant PTH2R. One highly bioactive analog,[ Bpa1,Nle8,18,Arg13,26,27,L-2-Nal23,Tyr34]PTH-(1–34)NH2 (Bpa1-PTH), was chosen for cross-linking studies. In addition, a PTH analog in which the photoreacive moiety is at the mid-region position 13 (K13) was demonstrated to be bioactive, then cross-linked to PTH2R. The minimal digestion-restricted domain containing the contact site (“contact domain”) for 125I-Bpa1-PTH is in the sixth transmembrane domain and part of the third extracellular loop, spanning residues Ser364-Met395 of the receptor. This domain was further confirmed and refined by cross-linking 125I-Bpa1-PTH to two receptor mutants, PTH2R[V380M]- and PTH2R[V380M,M395L]-receptors. Treatment of the cross-linked conjugates with cyanogen bromide identified a single amino acid (position 380) as the putative contact point. The contact domain for 125I-K13 is located in the N-terminal extracellular tail of the receptor (in the C-terminal portion) and spans Gln138-Met147. Further validation of this contact domain was accomplished by photocross-linking to point-mutated PTH2R[K137R] receptor. Previous studies in which PTH analogs were cross-linked to human PTH/PTHrP receptor (PTH1R) identified Met425 and Phe173-Met189 as the contact sites for Bpa1-PTH and K13, respectively. These studies demonstrate that both receptor subtypes, PTH1- and PTH2-receptors, use analogous sites for interaction with positions 1 and 13 in PTH." @default.
- W4230377260 created "2022-05-11" @default.
- W4230377260 creator A5083995805 @default.
- W4230377260 date "1999-09-01" @default.
- W4230377260 modified "2023-09-30" @default.
- W4230377260 title "Direct Identification of Two Contact Sites for Parathyroid Hormone (PTH) in the Novel PTH-2 Receptor using Photoaffinity Cross-Linking" @default.
- W4230377260 doi "https://doi.org/10.1210/en.140.9.4251" @default.
- W4230377260 hasPublicationYear "1999" @default.
- W4230377260 type Work @default.
- W4230377260 citedByCount "5" @default.
- W4230377260 countsByYear W42303772602015 @default.
- W4230377260 crossrefType "journal-article" @default.
- W4230377260 hasAuthorship W4230377260A5083995805 @default.
- W4230377260 hasBestOaLocation W42303772601 @default.
- W4230377260 hasConcept C104317684 @default.
- W4230377260 hasConcept C118892022 @default.
- W4230377260 hasConcept C121608353 @default.
- W4230377260 hasConcept C126322002 @default.
- W4230377260 hasConcept C167625842 @default.
- W4230377260 hasConcept C168557967 @default.
- W4230377260 hasConcept C170493617 @default.
- W4230377260 hasConcept C178790620 @default.
- W4230377260 hasConcept C185592680 @default.
- W4230377260 hasConcept C23589133 @default.
- W4230377260 hasConcept C2777093645 @default.
- W4230377260 hasConcept C2778581200 @default.
- W4230377260 hasConcept C2781208988 @default.
- W4230377260 hasConcept C28406088 @default.
- W4230377260 hasConcept C519063684 @default.
- W4230377260 hasConcept C530470458 @default.
- W4230377260 hasConcept C55493867 @default.
- W4230377260 hasConcept C71240020 @default.
- W4230377260 hasConcept C71924100 @default.
- W4230377260 hasConceptScore W4230377260C104317684 @default.
- W4230377260 hasConceptScore W4230377260C118892022 @default.
- W4230377260 hasConceptScore W4230377260C121608353 @default.
- W4230377260 hasConceptScore W4230377260C126322002 @default.
- W4230377260 hasConceptScore W4230377260C167625842 @default.
- W4230377260 hasConceptScore W4230377260C168557967 @default.
- W4230377260 hasConceptScore W4230377260C170493617 @default.
- W4230377260 hasConceptScore W4230377260C178790620 @default.
- W4230377260 hasConceptScore W4230377260C185592680 @default.
- W4230377260 hasConceptScore W4230377260C23589133 @default.
- W4230377260 hasConceptScore W4230377260C2777093645 @default.
- W4230377260 hasConceptScore W4230377260C2778581200 @default.
- W4230377260 hasConceptScore W4230377260C2781208988 @default.
- W4230377260 hasConceptScore W4230377260C28406088 @default.
- W4230377260 hasConceptScore W4230377260C519063684 @default.
- W4230377260 hasConceptScore W4230377260C530470458 @default.
- W4230377260 hasConceptScore W4230377260C55493867 @default.
- W4230377260 hasConceptScore W4230377260C71240020 @default.
- W4230377260 hasConceptScore W4230377260C71924100 @default.
- W4230377260 hasIssue "9" @default.
- W4230377260 hasLocation W42303772601 @default.
- W4230377260 hasOpenAccess W4230377260 @default.
- W4230377260 hasPrimaryLocation W42303772601 @default.
- W4230377260 hasRelatedWork W1535515186 @default.
- W4230377260 hasRelatedWork W1964908330 @default.
- W4230377260 hasRelatedWork W2047687741 @default.
- W4230377260 hasRelatedWork W2071832301 @default.
- W4230377260 hasRelatedWork W2134599993 @default.
- W4230377260 hasRelatedWork W3023510106 @default.
- W4230377260 hasRelatedWork W3176656515 @default.
- W4230377260 hasRelatedWork W3204300420 @default.
- W4230377260 hasRelatedWork W4243456438 @default.
- W4230377260 hasRelatedWork W4382314841 @default.
- W4230377260 hasVolume "140" @default.
- W4230377260 isParatext "false" @default.
- W4230377260 isRetracted "false" @default.
- W4230377260 workType "article" @default.