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- W4230385720 abstract "Abstract Background Recently, neural stem cell (NSC) therapy has shown promise for the treatment of many neurological diseases. Enhancing the quality of implanted cells and improving therapeutic efficacy are currently research hotspots. It has been reported that collagen sponge material provided sufficient room for cell growth in all directions, promoted the absorption of nutrients and removal of wastes. And also, the Rotary Cell Culture System (RCCS), which mimics the microgravity environment, can be used to culture cells for tissue engineering. Materials and methods We performed the mRNA and miRNA sequencing to elucidate the regulatory mechanism of NSCs cultured on the collagen sponge in RCCS system. The luciferase assay and Western blot revealed a direct regulatory role between let-7i-5p and NTRK3. And then, the neural differentiation maker Tuj1 and Map2 were detected by immunofluorescence staining. In the meantime, the migratory ability of NSCs was detected both in vitro and in spinal cord injury animals. Results In this study, we demonstrated that the expression of neurotrophic receptor tyrosine kinase 3 (NTRK3; also called TrkC) was elevated in NSCs cultured on collagen sponge in the RCCS system. Furthermore, increased NTRK3 expression was regulated by the downregulation of let-7i-5p. Compared to traditionally cultured NSCs, the NSCs cultured on collagen sponge in the RCCS system exhibited better neuronal differentiation and migratory ability, especially in the presence of NT-3. Conclusions As the biological properties and quality of transplanted cells are critical for therapeutic success, the RCCS system combined with collagen sponge culture system show promise for applications in clinical practice in the future." @default.
- W4230385720 created "2022-05-11" @default.
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- W4230385720 date "2020-12-01" @default.
- W4230385720 modified "2023-09-26" @default.
- W4230385720 title "The Rotary Cell Culture System increases NTRK3 Expression and Promotes Neuronal Differentiation and Migratory Ability of Neural Stem Cells Cultured on Collagen Sponge" @default.
- W4230385720 doi "https://doi.org/10.21203/rs.3.rs-115776/v1" @default.
- W4230385720 hasPublicationYear "2020" @default.
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