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- W4230484733 abstract "Abstract MIR29B2CHG/C1orf132 is the host gene for generating miR-29b2 and miR-29c. Here, we employed bioinformatics and experimental approaches to decipher expression of C1orf132 in breast cancer cells and tissues. Our data demonstrated a significant downregulation of C1orf132 in triple-negative breast cancer. We also predicted a putative promoter for the longer transcripts of C1orf132. The functionality of the distal promoter was confirmed by transfecting MCF7 cells with a C1orf132 promoter-GFP construct. Knocking-out the promoter by means of CRISPR/Cas9 approach revealed no expression alteration of neighboring genes, CD46 and CD34. However, the expression of miR-29c was reduced by half, suggesting an enhancer effect of the distal promoter on miR-29c generation. Furthermore, the promoter knock-out an elevation of migration ability in MCF12A edited cells. Moreover, the expressions of cell mobility genes e.g., CDH2, FGF2, FGFR1 and the stem cell and EMT-associated transcription factor ZEB1 were significantly elevated in edited cells. RNA sequencing data on the edited and unedited cells revealed many up- and down-regulated genes involved in various cellular pathways, including epithelial to mesenchymal transition and mammary gland development pathways. Altogether, we are reporting the existence of an additional/distal promoter with an enhancer effect on miR-29 generation and an inhibitory effect on cell migration." @default.
- W4230484733 created "2022-05-11" @default.
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- W4230484733 date "2021-01-29" @default.
- W4230484733 modified "2023-09-28" @default.
- W4230484733 title "Expression and Function of C1orf132 Long-Noncoding RNA, in Breast Cancer Cell Lines and Tissues" @default.
- W4230484733 doi "https://doi.org/10.21203/rs.3.rs-144862/v1" @default.
- W4230484733 hasPublicationYear "2021" @default.
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