Matches in SemOpenAlex for { <https://semopenalex.org/work/W4230505510> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W4230505510 endingPage "52" @default.
- W4230505510 startingPage "43" @default.
- W4230505510 abstract "Cryopreserved Chlamydomonas reinhardtii cultures remained viable when frozen by cooling slowly to −55°C, then plunging into liquid nitrogen for at least 1 day of storage. High viability (>40%) was retained when cultures contained 2–10% (v/v) methanol as a cryoprotective agent prior to freezing, while dimethyl sulphoxide was ineffective. However, methanol was lethal to cells in the presence of light. Frozen cultures became non-viable within 24 h when stored at −80°C, whereas those stored below −130°C remained viable for at least several months. Highest viability was attained in cultures that were frozen and stored at a low cell density. High viability also required that frozen cultures be warmed rapidly and the cryoprotective agent removed immediately thereafter in preparation for culturing in liquid or on solid medium. Individual cell viability was determined by measurements of colony counts after cell plating and by the penetration of Evans blue dye into non-viable cells. Viability in bulk culture was conveniently measured by comparing the rates of photosynthetic oxygen evolution, corrected for dark respiration, in previously frozen cultures with the corresponding rates in unfrozen controls. Cultures that had lost viability as measured by a decline in whole-cell photosynthetic oxygen evolution after freezing and thawing retained functional Photosystem II activity for some time thereafter, indicating that the loss in photosynthetic activity was due to some process other than photoinhibition of Photosystem II." @default.
- W4230505510 created "2022-05-11" @default.
- W4230505510 creator A5012804071 @default.
- W4230505510 creator A5032969091 @default.
- W4230505510 creator A5041467122 @default.
- W4230505510 date "1999-02-01" @default.
- W4230505510 modified "2023-10-05" @default.
- W4230505510 title "Cryopreservation of<i>Chlamydomonas reinhardtii</i>(Chlorophyta)" @default.
- W4230505510 doi "https://doi.org/10.1080/09670269910001736072" @default.
- W4230505510 hasPublicationYear "1999" @default.
- W4230505510 type Work @default.
- W4230505510 citedByCount "56" @default.
- W4230505510 countsByYear W42305055102012 @default.
- W4230505510 countsByYear W42305055102014 @default.
- W4230505510 countsByYear W42305055102015 @default.
- W4230505510 countsByYear W42305055102016 @default.
- W4230505510 countsByYear W42305055102017 @default.
- W4230505510 countsByYear W42305055102018 @default.
- W4230505510 countsByYear W42305055102019 @default.
- W4230505510 countsByYear W42305055102020 @default.
- W4230505510 countsByYear W42305055102021 @default.
- W4230505510 countsByYear W42305055102022 @default.
- W4230505510 countsByYear W42305055102023 @default.
- W4230505510 crossrefType "journal-article" @default.
- W4230505510 hasAuthorship W4230505510A5012804071 @default.
- W4230505510 hasAuthorship W4230505510A5032969091 @default.
- W4230505510 hasAuthorship W4230505510A5041467122 @default.
- W4230505510 hasConcept C104317684 @default.
- W4230505510 hasConcept C12554922 @default.
- W4230505510 hasConcept C143065580 @default.
- W4230505510 hasConcept C1491633281 @default.
- W4230505510 hasConcept C154828652 @default.
- W4230505510 hasConcept C172256273 @default.
- W4230505510 hasConcept C179933525 @default.
- W4230505510 hasConcept C183688256 @default.
- W4230505510 hasConcept C196843134 @default.
- W4230505510 hasConcept C2777550515 @default.
- W4230505510 hasConcept C2777563530 @default.
- W4230505510 hasConcept C2777727519 @default.
- W4230505510 hasConcept C53227056 @default.
- W4230505510 hasConcept C55493867 @default.
- W4230505510 hasConcept C559758991 @default.
- W4230505510 hasConcept C59822182 @default.
- W4230505510 hasConcept C80298142 @default.
- W4230505510 hasConcept C86803240 @default.
- W4230505510 hasConcept C95444343 @default.
- W4230505510 hasConceptScore W4230505510C104317684 @default.
- W4230505510 hasConceptScore W4230505510C12554922 @default.
- W4230505510 hasConceptScore W4230505510C143065580 @default.
- W4230505510 hasConceptScore W4230505510C1491633281 @default.
- W4230505510 hasConceptScore W4230505510C154828652 @default.
- W4230505510 hasConceptScore W4230505510C172256273 @default.
- W4230505510 hasConceptScore W4230505510C179933525 @default.
- W4230505510 hasConceptScore W4230505510C183688256 @default.
- W4230505510 hasConceptScore W4230505510C196843134 @default.
- W4230505510 hasConceptScore W4230505510C2777550515 @default.
- W4230505510 hasConceptScore W4230505510C2777563530 @default.
- W4230505510 hasConceptScore W4230505510C2777727519 @default.
- W4230505510 hasConceptScore W4230505510C53227056 @default.
- W4230505510 hasConceptScore W4230505510C55493867 @default.
- W4230505510 hasConceptScore W4230505510C559758991 @default.
- W4230505510 hasConceptScore W4230505510C59822182 @default.
- W4230505510 hasConceptScore W4230505510C80298142 @default.
- W4230505510 hasConceptScore W4230505510C86803240 @default.
- W4230505510 hasConceptScore W4230505510C95444343 @default.
- W4230505510 hasIssue "1" @default.
- W4230505510 hasLocation W42305055101 @default.
- W4230505510 hasOpenAccess W4230505510 @default.
- W4230505510 hasPrimaryLocation W42305055101 @default.
- W4230505510 hasRelatedWork W2019474790 @default.
- W4230505510 hasRelatedWork W2049307727 @default.
- W4230505510 hasRelatedWork W2160178141 @default.
- W4230505510 hasRelatedWork W2411003011 @default.
- W4230505510 hasRelatedWork W2702345470 @default.
- W4230505510 hasRelatedWork W3013016983 @default.
- W4230505510 hasRelatedWork W4230505510 @default.
- W4230505510 hasRelatedWork W4247123930 @default.
- W4230505510 hasRelatedWork W4291796127 @default.
- W4230505510 hasRelatedWork W4307968713 @default.
- W4230505510 hasVolume "34" @default.
- W4230505510 isParatext "false" @default.
- W4230505510 isRetracted "false" @default.
- W4230505510 workType "article" @default.